Analýza založená na mnohočetném protilátkovém souboru, detekci značením a rozlišení velikosti proteinů

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F09%3A00318349" target="_blank" >RIV/68378050:_____/09:00318349 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Antibody array analysis with label-based detection and resolution of protein size

Popis výsledku v původním jazyce

Proteins from different cellular compartments were labeled and separated by size exclusion chromatography into 20 fractions. The elution profiles were compiled to color maps across the size separation range (670-10kDa). A new solid phase designed for processing in microwell plates was developed to handle the large number of samples. Antibodies were bound to protein G-coupled microspheres surface-labeled with 300 combinations of four fluorescent dyes. Fluorescence from particle color codes and the protein label was measured by high speed flow cytometry. Cytoplasmic protein kinases, membrane proteins, cyclin-dependent kinases (cdks), cyclins and cdk-inhibitors, were analyzed; the results were compatible with their occurrence in complexes that vary with the cell cycle phase and subcellular localization. A two-dimensional platform extends the utility of antibody array analysis to studies of protein complexes.

Název v anglickém jazyce

Antibody array analysis with label-based detection and resolution of protein size

Popis výsledku anglicky

Proteins from different cellular compartments were labeled and separated by size exclusion chromatography into 20 fractions. The elution profiles were compiled to color maps across the size separation range (670-10kDa). A new solid phase designed for processing in microwell plates was developed to handle the large number of samples. Antibodies were bound to protein G-coupled microspheres surface-labeled with 300 combinations of four fluorescent dyes. Fluorescence from particle color codes and the protein label was measured by high speed flow cytometry. Cytoplasmic protein kinases, membrane proteins, cyclin-dependent kinases (cdks), cyclins and cdk-inhibitors, were analyzed; the results were compatible with their occurrence in complexes that vary with the cell cycle phase and subcellular localization. A two-dimensional platform extends the utility of antibody array analysis to studies of protein complexes.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

—

Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular and Cellular Proteomics

ISSN

1535-9484

e-ISSN

—

Svazek periodika

8

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CA - Kanada

Počet stran výsledku

13

Strana od-do

—

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—