BLM helicase measures DNA unwound before switching strands and hRPA promotes unwinding reinitiation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F09%3A00333639" target="_blank" >RIV/68378050:_____/09:00333639 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

BLM helicase measures DNA unwound before switching strands and hRPA promotes unwinding reinitiation

Popis výsledku v původním jazyce

Bloom syndrome (BS) is characterized by genomic instability and high predisposition to cancer. BLM, gene defective in BS, encodes a member of the RecQ family of 3´-5´ DNA helicases and is proposed to function in recombinational repair at DNA replication.We used single-molecule fluorescence resonance energy transfer microscopy to examine BLM behaviour on forked DNA substrates. BLM unwound individual DNA molecules repetitively, a short length unwound followed by rapid reannealing in successions. We showthat monomeric BLM can "measure" how many base pairs it has unwound, and after a critical length it reverses the unwinding through strand switching and translocating on the opposing strand. Repetitive unwinding persisted even in the presence of hRPA, andinteraction between wild-type BLM and hRPA was necessary for unwinding reinitiation on hRPA-coated DNA. These activities may facilitate BLM processing of stalled replication forks and illegitimately formed recombination intermediates.

Název v anglickém jazyce

BLM helicase measures DNA unwound before switching strands and hRPA promotes unwinding reinitiation

Popis výsledku anglicky

Bloom syndrome (BS) is characterized by genomic instability and high predisposition to cancer. BLM, gene defective in BS, encodes a member of the RecQ family of 3´-5´ DNA helicases and is proposed to function in recombinational repair at DNA replication.We used single-molecule fluorescence resonance energy transfer microscopy to examine BLM behaviour on forked DNA substrates. BLM unwound individual DNA molecules repetitively, a short length unwound followed by rapid reannealing in successions. We showthat monomeric BLM can "measure" how many base pairs it has unwound, and after a critical length it reverses the unwinding through strand switching and translocating on the opposing strand. Repetitive unwinding persisted even in the presence of hRPA, andinteraction between wild-type BLM and hRPA was necessary for unwinding reinitiation on hRPA-coated DNA. These activities may facilitate BLM processing of stalled replication forks and illegitimately formed recombination intermediates.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

EMBO Journal

ISSN

0261-4189

e-ISSN

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Svazek periodika

28

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

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Kód UT WoS článku

000263556200012

EID výsledku v databázi Scopus

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