Distinct regulatory roles of transforming growth factor-beta and interleukin-4 in the development and maintenance of natural and induced CD4+ CD25+ Foxp3+ regulatory T cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F09%3A00333972" target="_blank" >RIV/68378050:_____/09:00333972 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Distinct regulatory roles of transforming growth factor-beta and interleukin-4 in the development and maintenance of natural and induced CD4+ CD25+ Foxp3+ regulatory T cells

Popis výsledku v původním jazyce

The development and function of CD4+CD25+Foxp3+ regulatory T cells (Tregs) are strictly regulated by cytokines. Here we show that TGF-b and IL-4 play a crucial and antagonistic role in the development of Tregs and have distinct effects on maintenance ofnatural (nTregs) and antigen-induced (iTregs) Tregs. We demonstrated that CD4+CD25+Foxp3+ iTregs develop upon alloantigenic stimulation in the presence of TGF-b exclusively from CD4+CD25-Foxp3- precursors. Both induction of Foxp3 expression and Treg proliferation were prevented when the cells were stimulated in the presence of IL-4. IL-4 decreased the number of Foxp3+ cells in a population of iTregs while it substantially supported the survival of nTregs. iTregs inhibit cell proliferation comparably tonTregs and their suppressive capacity is not modulated by IL-4. These data suggest that TGF-b and IL-4 differentially regulate the development and maintenance of Tregs but have no influence on the suppressive activity of Tregs.

Název v anglickém jazyce

Distinct regulatory roles of transforming growth factor-beta and interleukin-4 in the development and maintenance of natural and induced CD4+ CD25+ Foxp3+ regulatory T cells

Popis výsledku anglicky

The development and function of CD4+CD25+Foxp3+ regulatory T cells (Tregs) are strictly regulated by cytokines. Here we show that TGF-b and IL-4 play a crucial and antagonistic role in the development of Tregs and have distinct effects on maintenance ofnatural (nTregs) and antigen-induced (iTregs) Tregs. We demonstrated that CD4+CD25+Foxp3+ iTregs develop upon alloantigenic stimulation in the presence of TGF-b exclusively from CD4+CD25-Foxp3- precursors. Both induction of Foxp3 expression and Treg proliferation were prevented when the cells were stimulated in the presence of IL-4. IL-4 decreased the number of Foxp3+ cells in a population of iTregs while it substantially supported the survival of nTregs. iTregs inhibit cell proliferation comparably tonTregs and their suppressive capacity is not modulated by IL-4. These data suggest that TGF-b and IL-4 differentially regulate the development and maintenance of Tregs but have no influence on the suppressive activity of Tregs.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Immunology

ISSN

0019-2805

e-ISSN

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Svazek periodika

128

Číslo periodika v rámci svazku

1 Suppl

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

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Kód UT WoS článku

000268703800052

EID výsledku v databázi Scopus

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