Catalytic activity of matrix metalloproteinase-19 is essential for tumor suppressor and anti-angiogenic activities in nasopharyngeal carcinoma

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F11%3A00354940" target="_blank" >RIV/68378050:_____/11:00354940 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1002/ijc.25855" target="_blank" >http://dx.doi.org/10.1002/ijc.25855</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/ijc.25855" target="_blank" >10.1002/ijc.25855</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Catalytic activity of matrix metalloproteinase-19 is essential for tumor suppressor and anti-angiogenic activities in nasopharyngeal carcinoma

Popis výsledku v původním jazyce

The association of matrix metalloproteinase-19 (MMP19) in the development of nasopharyngeal carcinoma (NPC) was identified from differential gene profiling, which showed MMP19 was one of the candidate genes down-regulated in the NPC cell lines. In this study, analysis showed MMP19 was down-regulated in all seven NPC cell lines and in 69.7% of primary NPC specimens. Allelic deletion and promoter hypermethylation contribute to MMP19 down-regulation. In the in vivo tumorigenicity assay, only the wild-type,but not mutant, MMP19 transfectants suppress tumor formation in nude mice. In the in vitro colony formation assay, WT MMP19 dramatically reduces colony-forming ability of NPC cell lines. In the tube formation assay secreted WT MMP19, but not mutant MMP19, induces reduction of tube-forming ability in endothelial cells with decreased vascular endothelial growth factor. The anti-angiogenic activity of WT MMP19 is correlated with suppression of tumor formation.

Název v anglickém jazyce

Catalytic activity of matrix metalloproteinase-19 is essential for tumor suppressor and anti-angiogenic activities in nasopharyngeal carcinoma

Popis výsledku anglicky

The association of matrix metalloproteinase-19 (MMP19) in the development of nasopharyngeal carcinoma (NPC) was identified from differential gene profiling, which showed MMP19 was one of the candidate genes down-regulated in the NPC cell lines. In this study, analysis showed MMP19 was down-regulated in all seven NPC cell lines and in 69.7% of primary NPC specimens. Allelic deletion and promoter hypermethylation contribute to MMP19 down-regulation. In the in vivo tumorigenicity assay, only the wild-type,but not mutant, MMP19 transfectants suppress tumor formation in nude mice. In the in vitro colony formation assay, WT MMP19 dramatically reduces colony-forming ability of NPC cell lines. In the tube formation assay secreted WT MMP19, but not mutant MMP19, induces reduction of tube-forming ability in endothelial cells with decreased vascular endothelial growth factor. The anti-angiogenic activity of WT MMP19 is correlated with suppression of tumor formation.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Cancer

ISSN

0020-7136

e-ISSN

—

Svazek periodika

129

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

12

Strana od-do

1826-1837

Kód UT WoS článku

000294224300004

EID výsledku v databázi Scopus

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