dsRNA expression in the mouse elicits RNAi in oocytes and low adenosine deamination in somatic cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F12%3A00371756" target="_blank" >RIV/68378050:_____/12:00371756 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1093/nar/gkr702" target="_blank" >http://dx.doi.org/10.1093/nar/gkr702</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/nar/gkr702" target="_blank" >10.1093/nar/gkr702</a>

Jazyk výsledku

angličtina

Název v původním jazyce

dsRNA expression in the mouse elicits RNAi in oocytes and low adenosine deamination in somatic cells

Popis výsledku v původním jazyce

Double-stranded RNA (dsRNA) can enter sequence-specific RNA interference (RNAi), sequence-independent interferon (IFN) response and editing by adenosine deaminases pathways. To study the fate of dsRNA in vivo, we used transgenic mice ubiquitously expressing from a promoter an mRNA with a long hairpin. The expressed dsRNA did not cause any developmental defects nor activated the IFN response, which was inducible only at high expression levels in cultured cells. dsRNA was poorly processed into siRNAs in somatic cells while robust RNAi effects were found in oocytes, suggesting that somatic cells lack some factor(s) facilitating siRNA biogenesis. Expressed dsRNA did not cause transcriptional silencing in trans. Analysis of RNA editing revealed an edited small fraction of long dsRNA. RNA editing did not prevent cytoplasmic localization nor processing into siRNAs. Thus, a long dsRNA structure is well tolerated in mammalian cells and is mainly causing a robust RNAi response in oocytes.

Název v anglickém jazyce

dsRNA expression in the mouse elicits RNAi in oocytes and low adenosine deamination in somatic cells

Popis výsledku anglicky

Double-stranded RNA (dsRNA) can enter sequence-specific RNA interference (RNAi), sequence-independent interferon (IFN) response and editing by adenosine deaminases pathways. To study the fate of dsRNA in vivo, we used transgenic mice ubiquitously expressing from a promoter an mRNA with a long hairpin. The expressed dsRNA did not cause any developmental defects nor activated the IFN response, which was inducible only at high expression levels in cultured cells. dsRNA was poorly processed into siRNAs in somatic cells while robust RNAi effects were found in oocytes, suggesting that somatic cells lack some factor(s) facilitating siRNA biogenesis. Expressed dsRNA did not cause transcriptional silencing in trans. Analysis of RNA editing revealed an edited small fraction of long dsRNA. RNA editing did not prevent cytoplasmic localization nor processing into siRNAs. Thus, a long dsRNA structure is well tolerated in mammalian cells and is mainly causing a robust RNAi response in oocytes.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA204%2F09%2F0085" target="_blank" >GA204/09/0085: RNA silencing a dlouhá dsRNA v savčích buňkách</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nucleic Acids Research

ISSN

0305-1048

e-ISSN

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Svazek periodika

40

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

15

Strana od-do

399-413

Kód UT WoS článku

000298733500043

EID výsledku v databázi Scopus

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