CRISPR-Induced Expression of N-Terminally Truncated Dicer in Mouse Cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F21%3A00555394" target="_blank" >RIV/68378050:_____/21:00555394 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.mdpi.com/2073-4425/12/4/540" target="_blank" >https://www.mdpi.com/2073-4425/12/4/540</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/genes12040540" target="_blank" >10.3390/genes12040540</a>

Jazyk výsledku

angličtina

Název v původním jazyce

CRISPR-Induced Expression of N-Terminally Truncated Dicer in Mouse Cells

Popis výsledku v původním jazyce

RNA interference (RNAi) designates sequence-specific mRNA degradation mediated by small RNAs generated from long double-stranded RNA (dsRNA) by RNase III Dicer. RNAi appears inactive in mammalian cells except for mouse oocytes, where high RNAi activity exists because of an N-terminally truncated Dicer isoform, denoted Dicer(O). Dicer(O) processes dsRNA into small RNAs more efficiently than the full-length Dicer expressed in somatic cells. Dicer(O) is expressed from an oocyte-specific promoter of retrotransposon origin, which is silenced in other cell types. In this work, we evaluated CRISPR-based strategies for epigenetic targeting of the endogenous Dicer gene to restore Dicer(O) expression and, consequently, RNAi. We show that reactivation of Dicer(O) expression can be achieved in mouse embryonic stem cells, but it is not sufficient to establish a robust canonical RNAi response.

Název v anglickém jazyce

CRISPR-Induced Expression of N-Terminally Truncated Dicer in Mouse Cells

Popis výsledku anglicky

RNA interference (RNAi) designates sequence-specific mRNA degradation mediated by small RNAs generated from long double-stranded RNA (dsRNA) by RNase III Dicer. RNAi appears inactive in mammalian cells except for mouse oocytes, where high RNAi activity exists because of an N-terminally truncated Dicer isoform, denoted Dicer(O). Dicer(O) processes dsRNA into small RNAs more efficiently than the full-length Dicer expressed in somatic cells. Dicer(O) is expressed from an oocyte-specific promoter of retrotransposon origin, which is silenced in other cell types. In this work, we evaluated CRISPR-based strategies for epigenetic targeting of the endogenous Dicer gene to restore Dicer(O) expression and, consequently, RNAi. We show that reactivation of Dicer(O) expression can be achieved in mouse embryonic stem cells, but it is not sufficient to establish a robust canonical RNAi response.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10603 - Genetics and heredity (medical genetics to be 3)

Projekt

<a href="/cs/project/GX20-03950X" target="_blank" >GX20-03950X: RNAi revival v savčím organismu</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Genes

ISSN

2073-4425

e-ISSN

2073-4425

Svazek periodika

12

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

11

Strana od-do

540

Kód UT WoS článku

000643040500001

EID výsledku v databázi Scopus

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