Administration of anti-CD25 mAb leads to impaired alpha-galactosylceramide-mediated induction of IFN-gamma production in a murine model

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F13%3A00395907" target="_blank" >RIV/68378050:_____/13:00395907 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.imbio.2012.10.012" target="_blank" >http://dx.doi.org/10.1016/j.imbio.2012.10.012</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.imbio.2012.10.012" target="_blank" >10.1016/j.imbio.2012.10.012</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Administration of anti-CD25 mAb leads to impaired alpha-galactosylceramide-mediated induction of IFN-gamma production in a murine model

Popis výsledku v původním jazyce

CD4(+)CD25(+)Foxp3(+) T regulatory cells (Tregs) and CD1d-restricted invariant natural killer T (iNKT) cells are two cell types that are known to regulate immune reactions. Depletion or inactivation of Tregs using specific anti-CD25 antibodies in combination with immunostimulation is an attractive modality especially in anti-tumour immunotherapy. However, CD25 is not expressed exclusively on Tregs but also on subpopulations of activated lymphocytes. Therefore, the modulatory effects of the specific anti-CD25 antibodies can also be partially attributed to their interactions with the effector cells. Here, the effector functions of iNKT cells were analysed in combination with anti-CD25 mAb PC61. Upon PC61 administration, alpha-galactosylceramide (alpha-GalCer)-mediated activation of iNKT cells resulted in decreased IFN-gamma but not IL-4 production. In order to determine whether mutual interactions between Tregs and iNKT cells take place, we compared IFN gamma production after alpha-GalCe

Název v anglickém jazyce

Administration of anti-CD25 mAb leads to impaired alpha-galactosylceramide-mediated induction of IFN-gamma production in a murine model

Popis výsledku anglicky

CD4(+)CD25(+)Foxp3(+) T regulatory cells (Tregs) and CD1d-restricted invariant natural killer T (iNKT) cells are two cell types that are known to regulate immune reactions. Depletion or inactivation of Tregs using specific anti-CD25 antibodies in combination with immunostimulation is an attractive modality especially in anti-tumour immunotherapy. However, CD25 is not expressed exclusively on Tregs but also on subpopulations of activated lymphocytes. Therefore, the modulatory effects of the specific anti-CD25 antibodies can also be partially attributed to their interactions with the effector cells. Here, the effector functions of iNKT cells were analysed in combination with anti-CD25 mAb PC61. Upon PC61 administration, alpha-galactosylceramide (alpha-GalCer)-mediated activation of iNKT cells resulted in decreased IFN-gamma but not IL-4 production. In order to determine whether mutual interactions between Tregs and iNKT cells take place, we compared IFN gamma production after alpha-GalCe

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Immunobiology

ISSN

0171-2985

e-ISSN

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Svazek periodika

218

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

9

Strana od-do

851-859

Kód UT WoS článku

000319486200003

EID výsledku v databázi Scopus

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