Expression of the chicken GDNF family receptor alpha-1 as a marker of spermatogonial stem cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F13%3A00422220" target="_blank" >RIV/68378050:_____/13:00422220 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/46356606:_____/13:#0000042

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.anireprosci.2013.08.006" target="_blank" >http://dx.doi.org/10.1016/j.anireprosci.2013.08.006</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.anireprosci.2013.08.006" target="_blank" >10.1016/j.anireprosci.2013.08.006</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Expression of the chicken GDNF family receptor alpha-1 as a marker of spermatogonial stem cells

Popis výsledku v původním jazyce

The identification, enrichment and subsequent isolation of spermatogonial stem cells (SSCs) are integral to the success of SCC transplants between fertile donor and sterilized recipient males. In birds generally and particularly in chicken, SSC-specifichas yet to be identified. The receptor for glial cell-derived neurotrophic factor (GDNF), i.e. GDNF family receptor alpha-1 (GFR alpha 1), has been identified as a potential marker for different mouse spermatogonial subtypes. In the present study, we characterized the chicken cGFR alpha 1 receptor and compared its predicted amino-acid sequence with mouse, rat and human GFR alpha 1 proteins. Using specific polyclonal mouse anti-cGFR alpha 1 serum, a total of 2.8% cells were recognized as cGFR alpha 1 positive among isolated testicular cells recovered from sexually mature cockerels. The percentages of cGFR alpha 1-positive testicular cells with haploid, diploid, tetraploid and SP DNA content were 1.6%, 2.5%, 393% and 76.8%, respectively.

Název v anglickém jazyce

Expression of the chicken GDNF family receptor alpha-1 as a marker of spermatogonial stem cells

Popis výsledku anglicky

The identification, enrichment and subsequent isolation of spermatogonial stem cells (SSCs) are integral to the success of SCC transplants between fertile donor and sterilized recipient males. In birds generally and particularly in chicken, SSC-specifichas yet to be identified. The receptor for glial cell-derived neurotrophic factor (GDNF), i.e. GDNF family receptor alpha-1 (GFR alpha 1), has been identified as a potential marker for different mouse spermatogonial subtypes. In the present study, we characterized the chicken cGFR alpha 1 receptor and compared its predicted amino-acid sequence with mouse, rat and human GFR alpha 1 proteins. Using specific polyclonal mouse anti-cGFR alpha 1 serum, a total of 2.8% cells were recognized as cGFR alpha 1 positive among isolated testicular cells recovered from sexually mature cockerels. The percentages of cGFR alpha 1-positive testicular cells with haploid, diploid, tetraploid and SP DNA content were 1.6%, 2.5%, 393% and 76.8%, respectively.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Animal Reproduction Science

ISSN

0378-4320

e-ISSN

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Svazek periodika

142

Číslo periodika v rámci svazku

1-2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

75-83

Kód UT WoS článku

000326558900011

EID výsledku v databázi Scopus

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