The first mouse mutants of D14Abb1e (Fam208a) show that it is critical for early development

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F14%3A00435131" target="_blank" >RIV/68378050:_____/14:00435131 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s00335-014-9516-0" target="_blank" >http://dx.doi.org/10.1007/s00335-014-9516-0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00335-014-9516-0" target="_blank" >10.1007/s00335-014-9516-0</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The first mouse mutants of D14Abb1e (Fam208a) show that it is critical for early development

Popis výsledku v původním jazyce

An ENU mutagenesis screen to identify novel epigenetic modifiers was established in mice carrying a multi-copy GFP transgene, which is expressed in a variegated manner in erythrocytes and is highly sensitive to epigenetic silencing. The screen has produced mouse mutants of both known modifiers of epigenetic state, such as Dnmt1 and Smarca5, and novel modifiers, such as Smchd1 and Rlf. Here we report two mouse lines generated from the screen, MommeD6 and MommeD20, with point mutations in D14Abb1e. Theseare the first mouse mutants of D14Abb1e (also known as Fam208a), a gene about which little is known. Heterozygous intercrosses show that homozygous mutants from both the MommeD6 and MommeD20 lines are not viable beyond gastrulation, demonstrating an important role for D14Abb1e in development. We demonstrate that haploinsufficiency for D14Abb1e effects transgene expression at the RNA level. Analysis of the predicted D14Abb1e protein sequence reveals that it contains putative nuclear local

Název v anglickém jazyce

The first mouse mutants of D14Abb1e (Fam208a) show that it is critical for early development

Popis výsledku anglicky

An ENU mutagenesis screen to identify novel epigenetic modifiers was established in mice carrying a multi-copy GFP transgene, which is expressed in a variegated manner in erythrocytes and is highly sensitive to epigenetic silencing. The screen has produced mouse mutants of both known modifiers of epigenetic state, such as Dnmt1 and Smarca5, and novel modifiers, such as Smchd1 and Rlf. Here we report two mouse lines generated from the screen, MommeD6 and MommeD20, with point mutations in D14Abb1e. Theseare the first mouse mutants of D14Abb1e (also known as Fam208a), a gene about which little is known. Heterozygous intercrosses show that homozygous mutants from both the MommeD6 and MommeD20 lines are not viable beyond gastrulation, demonstrating an important role for D14Abb1e in development. We demonstrate that haploinsufficiency for D14Abb1e effects transgene expression at the RNA level. Analysis of the predicted D14Abb1e protein sequence reveals that it contains putative nuclear local

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Mammalian Genome

ISSN

0938-8990

e-ISSN

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Svazek periodika

25

Číslo periodika v rámci svazku

7-8

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

293-303

Kód UT WoS článku

000339875100002

EID výsledku v databázi Scopus

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