L-plastin is involved in NKG2D recruitment into lipid rafts and NKG2D-mediated NK cell migration

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F14%3A00436786" target="_blank" >RIV/68378050:_____/14:00436786 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1189/jlb.2A1013-564R" target="_blank" >http://dx.doi.org/10.1189/jlb.2A1013-564R</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1189/jlb.2A1013-564R" target="_blank" >10.1189/jlb.2A1013-564R</a>

Jazyk výsledku

angličtina

Název v původním jazyce

L-plastin is involved in NKG2D recruitment into lipid rafts and NKG2D-mediated NK cell migration

Popis výsledku v původním jazyce

Membrane rafts are microdomains of the plasma membrane that have multiple biological functions. The involvement of these structures in the biology of T cells, namely in signal transduction by the TCR, has been widely studied. However, the role of membrane rafts in immunoreceptor signaling in NK cells is less well known. We studied the distribution of the activating NKG2D receptor in lipid rafts by isolating DRMs in a sucrose density gradient or by raft fractionation by ?-OG-selective solubility in the NKL cell line. We found that the NKG2D-DAP10 complex and pVav are recruited into rafts upon receptor stimulation. Qualitative proteomic analysis of these fractions showed that the actin cytoskeleton is involved in this process. In particular, we found that the actin-bundling protein L-plastin plays an important role in the clustering of NKG2D into lipid rafts. Moreover, coengagement of the inhibitory receptor NKG2A partially disrupted NKG2D recruitment into rafts. Furthermore, we demonstr

Název v anglickém jazyce

L-plastin is involved in NKG2D recruitment into lipid rafts and NKG2D-mediated NK cell migration

Popis výsledku anglicky

Membrane rafts are microdomains of the plasma membrane that have multiple biological functions. The involvement of these structures in the biology of T cells, namely in signal transduction by the TCR, has been widely studied. However, the role of membrane rafts in immunoreceptor signaling in NK cells is less well known. We studied the distribution of the activating NKG2D receptor in lipid rafts by isolating DRMs in a sucrose density gradient or by raft fractionation by ?-OG-selective solubility in the NKL cell line. We found that the NKG2D-DAP10 complex and pVav are recruited into rafts upon receptor stimulation. Qualitative proteomic analysis of these fractions showed that the actin cytoskeleton is involved in this process. In particular, we found that the actin-bundling protein L-plastin plays an important role in the clustering of NKG2D into lipid rafts. Moreover, coengagement of the inhibitory receptor NKG2A partially disrupted NKG2D recruitment into rafts. Furthermore, we demonstr

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GBP302%2F12%2FG101" target="_blank" >GBP302/12/G101: Molekulární mechanismy signalizace receptory leukocytů - jejich role ve zdraví a nemocích.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Leukocyte Biology

ISSN

0741-5400

e-ISSN

—

Svazek periodika

96

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

437-45

Kód UT WoS článku

000340829400010

EID výsledku v databázi Scopus

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