Genetic interaction between Pax6 and ?-catenin in the developing retinal pigment epithelium

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F15%3A00455800" target="_blank" >RIV/68378050:_____/15:00455800 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s00427-015-0493-4" target="_blank" >http://dx.doi.org/10.1007/s00427-015-0493-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00427-015-0493-4" target="_blank" >10.1007/s00427-015-0493-4</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Genetic interaction between Pax6 and ?-catenin in the developing retinal pigment epithelium

Popis výsledku v původním jazyce

Wnt/beta-catenin signaling plays an essential role in the retinal pigment epithelium (RPE) determination. Since activity of Pax6 (together with Pax2) is also required for the RPE determination, we investigated a possible genetic interaction between Pax6and Wnt/beta-catenin signaling pathway by analyzing Pax6, beta-catenin, and Pax6/beta-catenin conditional knockout mice. Although Pax6 inactivation alone had no impact on initial specification determined by the expression of Mitf and Otx2, melanin pigmentation was reduced in the RPE. This suggests that along with Mitf and Otx2, Pax6 is required for the full differentiation of RPE. Reporter gene assays in vitro suggest that hypopigmentation is at least in part due to the direct regulation of genes encoding enzymes involved in melanin synthesis by Pax6, Mitf, and beta-catenin. The RPE of a beta-catenin/Pax6 double mutant was differentiated into the neural retina; however, the tissue was thinner than that of the conditional beta-catenin mu

Název v anglickém jazyce

Genetic interaction between Pax6 and ?-catenin in the developing retinal pigment epithelium

Popis výsledku anglicky

Wnt/beta-catenin signaling plays an essential role in the retinal pigment epithelium (RPE) determination. Since activity of Pax6 (together with Pax2) is also required for the RPE determination, we investigated a possible genetic interaction between Pax6and Wnt/beta-catenin signaling pathway by analyzing Pax6, beta-catenin, and Pax6/beta-catenin conditional knockout mice. Although Pax6 inactivation alone had no impact on initial specification determined by the expression of Mitf and Otx2, melanin pigmentation was reduced in the RPE. This suggests that along with Mitf and Otx2, Pax6 is required for the full differentiation of RPE. Reporter gene assays in vitro suggest that hypopigmentation is at least in part due to the direct regulation of genes encoding enzymes involved in melanin synthesis by Pax6, Mitf, and beta-catenin. The RPE of a beta-catenin/Pax6 double mutant was differentiated into the neural retina; however, the tissue was thinner than that of the conditional beta-catenin mu

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Development Genes and Evolution

ISSN

0949-944X

e-ISSN

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Svazek periodika

225

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

8

Strana od-do

121-128

Kód UT WoS článku

000351605300006

EID výsledku v databázi Scopus

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