ZNF143 protein is an important regulator of the myeloid transcription factor C/EBP
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F17%3A00486300" target="_blank" >RIV/68378050:_____/17:00486300 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216208:11130/17:10373485
Výsledek na webu
<a href="http://dx.doi.org/10.1074/jbc.M117.811109" target="_blank" >http://dx.doi.org/10.1074/jbc.M117.811109</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1074/jbc.M117.811109" target="_blank" >10.1074/jbc.M117.811109</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
ZNF143 protein is an important regulator of the myeloid transcription factor C/EBP
Popis výsledku v původním jazyce
The transcription factor C/EBP is essential for myeloid differentiation and is frequently dysregulated in acute myeloid leukemia. Although studied extensively, the precise regulation of its gene by upstream factors has remained largely elusive. Here, we investigated its transcriptional activation during myeloid differentiation. We identified an evolutionarily conserved octameric sequence, CCCAGCAG, approximate to 100 bases upstream of the CEBPA transcription start site, and demonstrated through mutational analysis that this sequence is crucial for C/EBP expression. This sequence is present in the genes encoding C/EBP in humans, rodents, chickens, and frogs and is also present in the promoters of other C/EBP family members. We identified that ZNF143, the human homolog of the Xenopus transcriptional activator STAF, specifically binds to this 8-bp sequence to activate C/EBP expression in myeloid cells through a mechanism that is distinct from that observed in liver cells and adipocytes. Altogether, our data suggest that ZNF143 plays an important role in the expression of C/EBP in myeloid cells.
Název v anglickém jazyce
ZNF143 protein is an important regulator of the myeloid transcription factor C/EBP
Popis výsledku anglicky
The transcription factor C/EBP is essential for myeloid differentiation and is frequently dysregulated in acute myeloid leukemia. Although studied extensively, the precise regulation of its gene by upstream factors has remained largely elusive. Here, we investigated its transcriptional activation during myeloid differentiation. We identified an evolutionarily conserved octameric sequence, CCCAGCAG, approximate to 100 bases upstream of the CEBPA transcription start site, and demonstrated through mutational analysis that this sequence is crucial for C/EBP expression. This sequence is present in the genes encoding C/EBP in humans, rodents, chickens, and frogs and is also present in the promoters of other C/EBP family members. We identified that ZNF143, the human homolog of the Xenopus transcriptional activator STAF, specifically binds to this 8-bp sequence to activate C/EBP expression in myeloid cells through a mechanism that is distinct from that observed in liver cells and adipocytes. Altogether, our data suggest that ZNF143 plays an important role in the expression of C/EBP in myeloid cells.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
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OECD FORD obor
10601 - Cell biology
Návaznosti výsledku
Projekt
—
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2017
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Biological Chemistry
ISSN
0021-9258
e-ISSN
—
Svazek periodika
292
Číslo periodika v rámci svazku
46
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
13
Strana od-do
18924-18936
Kód UT WoS článku
000415848000017
EID výsledku v databázi Scopus
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