The mannose 6-phosphate/insulin-like growth factor 2 receptor mediates plasminogen-induced efferocytosis
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F19%3A00523092" target="_blank" >RIV/68378050:_____/19:00523092 - isvavai.cz</a>
Výsledek na webu
<a href="https://jlb.onlinelibrary.wiley.com/doi/full/10.1002/JLB.1AB0417-160RR" target="_blank" >https://jlb.onlinelibrary.wiley.com/doi/full/10.1002/JLB.1AB0417-160RR</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/JLB.1AB0417-160RR" target="_blank" >10.1002/JLB.1AB0417-160RR</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
The mannose 6-phosphate/insulin-like growth factor 2 receptor mediates plasminogen-induced efferocytosis
Popis výsledku v původním jazyce
The plasminogen system is harnessed in a wide variety of physiological processes, such as fibrinolysis, cell migration, or efferocytosis, and accordingly, it is essential upon inflammation, tissue remodeling, wound healing, and for homeostatic maintenance in general. Previously, we identified a plasminogen receptor in the mannose 6-phosphate/insulin-like growth factor 2 receptor (M6P/IGF2R, CD222). Here, we demonstrate by means of genetic knockdown, knockout, and rescue approaches combined with functional studies that M6P/IGF2R is up-regulated on the surface of macrophages, recognizes plasminogen exposed on the surface of apoptotic cells, and mediates plasminogen-induced efferocytosis. The level of uptake of plasminogen-coated apoptotic cells inversely correlates with the TNF-alpha production by phagocytes indicating tissue clearance without inflammation by this mechanism. Our results reveal an up-to-now undetermined function of M6P/IGF2R in clearance of apoptotic cells, which is crucial for tissue homeostasis.
Název v anglickém jazyce
The mannose 6-phosphate/insulin-like growth factor 2 receptor mediates plasminogen-induced efferocytosis
Popis výsledku anglicky
The plasminogen system is harnessed in a wide variety of physiological processes, such as fibrinolysis, cell migration, or efferocytosis, and accordingly, it is essential upon inflammation, tissue remodeling, wound healing, and for homeostatic maintenance in general. Previously, we identified a plasminogen receptor in the mannose 6-phosphate/insulin-like growth factor 2 receptor (M6P/IGF2R, CD222). Here, we demonstrate by means of genetic knockdown, knockout, and rescue approaches combined with functional studies that M6P/IGF2R is up-regulated on the surface of macrophages, recognizes plasminogen exposed on the surface of apoptotic cells, and mediates plasminogen-induced efferocytosis. The level of uptake of plasminogen-coated apoptotic cells inversely correlates with the TNF-alpha production by phagocytes indicating tissue clearance without inflammation by this mechanism. Our results reveal an up-to-now undetermined function of M6P/IGF2R in clearance of apoptotic cells, which is crucial for tissue homeostasis.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
30102 - Immunology
Návaznosti výsledku
Projekt
—
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2019
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Leukocyte Biology
ISSN
0741-5400
e-ISSN
—
Svazek periodika
105
Číslo periodika v rámci svazku
3
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
12
Strana od-do
519-530
Kód UT WoS článku
000459489900009
EID výsledku v databázi Scopus
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