Specific targeting and off-targeting in RNAi and miRNA pathways

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F19%3A00523494" target="_blank" >RIV/68378050:_____/19:00523494 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Specific targeting and off-targeting in RNAi and miRNA pathways

Popis výsledku v původním jazyce

This review systematically covers sequence-specific gene regulation by miRNAs and dsRNA-derived siRNAs in animals and plants from the perspective of target RNA recognition, potential for non-target (off-target) effects, and reliable determination of biological effects of small RNAs in animals and plants. I will review sequence complementarity between target RNA and small RNA (siRNA or miRNA), including tolerance to mismatches, parameters influencing sequence complementarity (and target recognition and repression) and discuss specificity of targeting by miRNAs and off-targeting by siRNAs. In addition, I will discuss reliable identification of target RNAs (and, eventually, biological effects). Accordingly, the text is divided into the four sections: (I) Small RNA:target RNA base pairing, (II) Other key factors influencing target recognition and repression, (III) Off-targeting – causes and remedies, (III) Small RNA target identification.

Název v anglickém jazyce

Specific targeting and off-targeting in RNAi and miRNA pathways

Popis výsledku anglicky

This review systematically covers sequence-specific gene regulation by miRNAs and dsRNA-derived siRNAs in animals and plants from the perspective of target RNA recognition, potential for non-target (off-target) effects, and reliable determination of biological effects of small RNAs in animals and plants. I will review sequence complementarity between target RNA and small RNA (siRNA or miRNA), including tolerance to mismatches, parameters influencing sequence complementarity (and target recognition and repression) and discuss specificity of targeting by miRNAs and off-targeting by siRNAs. In addition, I will discuss reliable identification of target RNAs (and, eventually, biological effects). Accordingly, the text is divided into the four sections: (I) Small RNA:target RNA base pairing, (II) Other key factors influencing target recognition and repression, (III) Off-targeting – causes and remedies, (III) Small RNA target identification.

Druh

C - Kapitola v odborné knize

CEP obor

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OECD FORD obor

10602 - Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology

Projekt

<a href="/cs/project/LO1220" target="_blank" >LO1220: CZ-OPENSCREEN: Národní infrastruktura chemické biologie</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Introduction to RNAi and miRNA pathways

ISBN

978-80-246-4372-4

Počet stran výsledku

38

Strana od-do

1-339

Počet stran knihy

339

Název nakladatele

Karolinum

Místo vydání

Praha

Kód UT WoS kapitoly

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