Distinct populations of crypt-associated fibroblasts act as signaling hubs to control colon homeostasis
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F20%3A00538133" target="_blank" >RIV/68378050:_____/20:00538133 - isvavai.cz</a>
Výsledek na webu
<a href="https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3001032" target="_blank" >https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3001032</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1371/journal.pbio.3001032" target="_blank" >10.1371/journal.pbio.3001032</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Distinct populations of crypt-associated fibroblasts act as signaling hubs to control colon homeostasis
Popis výsledku v původním jazyce
Despite recent progress in recognizing the importance of mesenchymal cells for the homeostasis of the intestinal system, the current picture of how these cells communicate with the associated epithelial layer remains unclear. To describe the relevant cell populations in an unbiased manner, we carried out a single-cell transcriptome analysis of the adult murine colon, producing a high-quality atlas of matched colonic epithelium and mesenchyme. We identify two crypt-associated colonic fibroblast populations that are demarcated by different strengths of platelet-derived growth factor receptor A (Pdgfra) expression. Crypt-bottom fibroblasts (CBFs), close to the intestinal stem cells, express low levels of Pdgfra and secrete canonical Wnt ligands, Wnt potentiators, and bone morphogenetic protein (Bmp) inhibitors. Crypt-top fibroblasts (CTFs) exhibit high Pdgfra levels and secrete noncanonical Wnts and Bmp ligands. While the Pdgfra(low) cells maintain intestinal stem cell proliferation, the Pdgfra(high) cells induce differentiation of the epithelial cells. Our findings enhance our understanding of the crosstalk between various colonic epithelial cells and their associated mesenchymal signaling hubs along the crypt axis-placing differential Pdgfra expression levels in the spotlight of intestinal fibroblast identity.
Název v anglickém jazyce
Distinct populations of crypt-associated fibroblasts act as signaling hubs to control colon homeostasis
Popis výsledku anglicky
Despite recent progress in recognizing the importance of mesenchymal cells for the homeostasis of the intestinal system, the current picture of how these cells communicate with the associated epithelial layer remains unclear. To describe the relevant cell populations in an unbiased manner, we carried out a single-cell transcriptome analysis of the adult murine colon, producing a high-quality atlas of matched colonic epithelium and mesenchyme. We identify two crypt-associated colonic fibroblast populations that are demarcated by different strengths of platelet-derived growth factor receptor A (Pdgfra) expression. Crypt-bottom fibroblasts (CBFs), close to the intestinal stem cells, express low levels of Pdgfra and secrete canonical Wnt ligands, Wnt potentiators, and bone morphogenetic protein (Bmp) inhibitors. Crypt-top fibroblasts (CTFs) exhibit high Pdgfra levels and secrete noncanonical Wnts and Bmp ligands. While the Pdgfra(low) cells maintain intestinal stem cell proliferation, the Pdgfra(high) cells induce differentiation of the epithelial cells. Our findings enhance our understanding of the crosstalk between various colonic epithelial cells and their associated mesenchymal signaling hubs along the crypt axis-placing differential Pdgfra expression levels in the spotlight of intestinal fibroblast identity.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
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OECD FORD obor
10608 - Biochemistry and molecular biology
Návaznosti výsledku
Projekt
<a href="/cs/project/GA18-21466S" target="_blank" >GA18-21466S: Ligandy Wnt a buňky produkující tyto ligandy při obnově střevního epitelu, jeho regeneraci a v nádorech tlustého střeva mimo mutaci indukující nádor</a><br>
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2020
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
P L o S Biology
ISSN
1544-9173
e-ISSN
—
Svazek periodika
18
Číslo periodika v rámci svazku
12
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
20
Strana od-do
e3001032
Kód UT WoS článku
000600098800002
EID výsledku v databázi Scopus
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