Human galectin-3: Molecular switch of gene expression in dermal fibroblasts in vitro and of skin collagen organization in open wounds and tensile strength in incisions in vivo
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F21%3A00544840" target="_blank" >RIV/68378050:_____/21:00544840 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216208:11120/21:43920839 RIV/00216208:11110/21:10419243
Výsledek na webu
<a href="https://www.spandidos-publications.com/10.3892/mmr.2020.11738" target="_blank" >https://www.spandidos-publications.com/10.3892/mmr.2020.11738</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3892/mmr.2020.11738" target="_blank" >10.3892/mmr.2020.11738</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Human galectin-3: Molecular switch of gene expression in dermal fibroblasts in vitro and of skin collagen organization in open wounds and tensile strength in incisions in vivo
Popis výsledku v původním jazyce
Understanding the molecular and cellular processes in skin wound healing can pave the way for devising innovative concepts by turning the identified natural effectors into therapeutic tools. Based on the concept of broad-scale engagement of members of the family of galactoside-binding lectins (galectins) in pathophysiological processes, such as cancer or tissue repair/regeneration, the present study investigated the potential of galectins-1 (Gal-1) and3 (Gal-3) in wound healing. Human dermal fibroblasts, which are key cells involved in skin wound healing, responded to galectin exposure (Gal-1 at 300 or Gal-3 at 600 ng/ml) with selective changes in gene expression among a panel of 84 wound-healing-related genes, as well as remodeling of the extracellular matrix. In the case of Gal-3, positive expression of Ki67 and cell number increased when using a decellularized matrix produced by Gal-3-treated fibroblasts as substrate for culture of interfollicular keratinocytes. In vivo wounds were topically treated with 20 ng/ml Gal-1 or3, and collagen score was found to be elevated in excisional wound repair in rats treated with Gal-3. The tensile strength measured in incisions was significantly increased from 79.5 +/- 17.5 g/mm(2) in controls to 103.1 +/- 21.4 g/mm(2) after 21 days of healing. These data warrant further testing mixtures of galectins and other types of compounds, for example a combination of galectins and TGF-beta 1.
Název v anglickém jazyce
Human galectin-3: Molecular switch of gene expression in dermal fibroblasts in vitro and of skin collagen organization in open wounds and tensile strength in incisions in vivo
Popis výsledku anglicky
Understanding the molecular and cellular processes in skin wound healing can pave the way for devising innovative concepts by turning the identified natural effectors into therapeutic tools. Based on the concept of broad-scale engagement of members of the family of galactoside-binding lectins (galectins) in pathophysiological processes, such as cancer or tissue repair/regeneration, the present study investigated the potential of galectins-1 (Gal-1) and3 (Gal-3) in wound healing. Human dermal fibroblasts, which are key cells involved in skin wound healing, responded to galectin exposure (Gal-1 at 300 or Gal-3 at 600 ng/ml) with selective changes in gene expression among a panel of 84 wound-healing-related genes, as well as remodeling of the extracellular matrix. In the case of Gal-3, positive expression of Ki67 and cell number increased when using a decellularized matrix produced by Gal-3-treated fibroblasts as substrate for culture of interfollicular keratinocytes. In vivo wounds were topically treated with 20 ng/ml Gal-1 or3, and collagen score was found to be elevated in excisional wound repair in rats treated with Gal-3. The tensile strength measured in incisions was significantly increased from 79.5 +/- 17.5 g/mm(2) in controls to 103.1 +/- 21.4 g/mm(2) after 21 days of healing. These data warrant further testing mixtures of galectins and other types of compounds, for example a combination of galectins and TGF-beta 1.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
30204 - Oncology
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2021
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Molecular Medicine Reports
ISSN
1791-2997
e-ISSN
1791-3004
Svazek periodika
23
Číslo periodika v rámci svazku
2
Stát vydavatele periodika
GR - Řecká republika
Počet stran výsledku
11
Strana od-do
99
Kód UT WoS článku
000601339700001
EID výsledku v databázi Scopus
—