Demystifying emerging bulk RNA-Seq applications: the application and utility of bioinformatic methodology

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F21%3A00555595" target="_blank" >RIV/68378050:_____/21:00555595 - isvavai.cz</a>

Výsledek na webu

<a href="https://academic.oup.com/bib/article-abstract/22/6/bbab259/6330938?redirectedFrom=fulltext&login=false" target="_blank" >https://academic.oup.com/bib/article-abstract/22/6/bbab259/6330938?redirectedFrom=fulltext&login=false</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/bib/bbab259" target="_blank" >10.1093/bib/bbab259</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Demystifying emerging bulk RNA-Seq applications: the application and utility of bioinformatic methodology

Popis výsledku v původním jazyce

Significant innovations in next-generation sequencing techniques and bioinformatics tools have impacted our appreciation and understanding of RNA. Practical RNA sequencing (RNA-Seq) applications have evolved in conjunction with sequence technology and bioinformatic tools advances. In most projects, bulk RNA-Seq data is used to measure gene expression patterns, isoform expression, alternative splicing and single-nucleotide polymorphisms. However, RNA-Seq holds far more hidden biological information including details of copy number alteration, microbial contamination, transposable elements, cell type (deconvolution) and the presence of neoantigens. Recent novel and advanced bioinformatic algorithms developed the capacity to retrieve this information from bulk RNA-Seq data, thus broadening its scope. The focus of this review is to comprehend the emerging bulk RNA-Seq-based analyses, emphasizing less familiar and underused applications. In doing so, we highlight the power of bulk RNA-Seq in providing biological insights.

Název v anglickém jazyce

Demystifying emerging bulk RNA-Seq applications: the application and utility of bioinformatic methodology

Popis výsledku anglicky

Significant innovations in next-generation sequencing techniques and bioinformatics tools have impacted our appreciation and understanding of RNA. Practical RNA sequencing (RNA-Seq) applications have evolved in conjunction with sequence technology and bioinformatic tools advances. In most projects, bulk RNA-Seq data is used to measure gene expression patterns, isoform expression, alternative splicing and single-nucleotide polymorphisms. However, RNA-Seq holds far more hidden biological information including details of copy number alteration, microbial contamination, transposable elements, cell type (deconvolution) and the presence of neoantigens. Recent novel and advanced bioinformatic algorithms developed the capacity to retrieve this information from bulk RNA-Seq data, thus broadening its scope. The focus of this review is to comprehend the emerging bulk RNA-Seq-based analyses, emphasizing less familiar and underused applications. In doing so, we highlight the power of bulk RNA-Seq in providing biological insights.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/GA20-04099S" target="_blank" >GA20-04099S: Molekulární charakterizace retinálních a cerebelárních defektů způsobených mutacemi sestřihového faktoru Prpf8</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Briefings in Bioinformatics

ISSN

1467-5463

e-ISSN

1477-4054

Svazek periodika

22

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

16

Strana od-do

bbab259

Kód UT WoS článku

000733325700129

EID výsledku v databázi Scopus

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