Nucleoporin TPR Affects C2C12 Myogenic Differentiation via Regulation of Myh4 Expression

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F21%3A00555598" target="_blank" >RIV/68378050:_____/21:00555598 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11110/21:10428127 RIV/68407700:21230/21:00350044 RIV/00216208:11130/21:10428127 RIV/00216208:11310/21:10428127

Výsledek na webu

<a href="https://www.mdpi.com/2073-4409/10/6/1271" target="_blank" >https://www.mdpi.com/2073-4409/10/6/1271</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/cells10061271" target="_blank" >10.3390/cells10061271</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Nucleoporin TPR Affects C2C12 Myogenic Differentiation via Regulation of Myh4 Expression

Popis výsledku v původním jazyce

The nuclear pore complex (NPC) has emerged as a hub for the transcriptional regulation of a subset of genes, and this type of regulation plays an important role during differentiation. Nucleoporin TPR forms the nuclear basket of the NPC and is crucial for the enrichment of open chromatin around NPCs. TPR has been implicated in the regulation of transcription, however, the role of TPR in gene expression and cell differentiation has not been described. Here we show that depletion of TPR results in an aberrant morphology of murine proliferating C2C12 myoblasts (MBs) and differentiated C2C12 myotubes (MTs). The ChIP-Seq data revealed that TPR binds to genes linked to muscle formation and function, such as myosin heavy chain (Myh4), myocyte enhancer factor 2C (Mef2C) and a majority of olfactory receptor (Olfr) genes. We further show that TPR, possibly via lysine-specific demethylase 1 (LSD1), promotes the expression of Myh4 and Olfr376, but not Mef2C. This provides a novel insight into the mechanism of myogenesis, however, more evidence is needed to fully elucidate the mechanism by which TPR affects specific myogenic genes.

Název v anglickém jazyce

Nucleoporin TPR Affects C2C12 Myogenic Differentiation via Regulation of Myh4 Expression

Popis výsledku anglicky

The nuclear pore complex (NPC) has emerged as a hub for the transcriptional regulation of a subset of genes, and this type of regulation plays an important role during differentiation. Nucleoporin TPR forms the nuclear basket of the NPC and is crucial for the enrichment of open chromatin around NPCs. TPR has been implicated in the regulation of transcription, however, the role of TPR in gene expression and cell differentiation has not been described. Here we show that depletion of TPR results in an aberrant morphology of murine proliferating C2C12 myoblasts (MBs) and differentiated C2C12 myotubes (MTs). The ChIP-Seq data revealed that TPR binds to genes linked to muscle formation and function, such as myosin heavy chain (Myh4), myocyte enhancer factor 2C (Mef2C) and a majority of olfactory receptor (Olfr) genes. We further show that TPR, possibly via lysine-specific demethylase 1 (LSD1), promotes the expression of Myh4 and Olfr376, but not Mef2C. This provides a novel insight into the mechanism of myogenesis, however, more evidence is needed to fully elucidate the mechanism by which TPR affects specific myogenic genes.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10601 - Cell biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cells

ISSN

2073-4409

e-ISSN

2073-4409

Svazek periodika

10

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

21

Strana od-do

1271

Kód UT WoS článku

000665511800001

EID výsledku v databázi Scopus

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