The Discovery of Chicken Foxp3 Demands Redefinition of Avian Regulatory T Cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F22%3A00555980" target="_blank" >RIV/68378050:_____/22:00555980 - isvavai.cz</a>

Výsledek na webu

<a href="https://journals.aai.org/jimmunol/article/208/5/1128/234858/The-Discovery-of-Chicken-Foxp3-Demands" target="_blank" >https://journals.aai.org/jimmunol/article/208/5/1128/234858/The-Discovery-of-Chicken-Foxp3-Demands</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4049/jimmunol.2000301" target="_blank" >10.4049/jimmunol.2000301</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The Discovery of Chicken Foxp3 Demands Redefinition of Avian Regulatory T Cells

Popis výsledku v původním jazyce

Since the publication of the first chicken genome sequence, we have encountered genes playing key roles in mammalian immunology, but being seemingly absent in birds. One of those was, until recently, Foxp3, the master transcription factor of regulatory T cells in mammals. Therefore, avian regulatory T cell research is still poorly standardized. In this study we identify a chicken ortholog of Foxp3. We prove sequence homology with known mammalian and sauropsid sequences, but also reveal differences in major domains. Expression profiling shows an association of Foxp3 and CD25 expression levels in CD4(+) CD25(+) peripheral T cells and identifies a CD4(-) CD25(+) Foxp3(high) subset of thymic lymphocytes that likely represents yet undescribed avian regulatory T precursor cells. We conclude that Foxp3 is existent in chickens and that it shares certain functional characteristics with its mammalian ortholog. Nevertheless, pathways for regulatory T cell development and Foxp3 function are likely to differ between mammals and birds. The identification and characterization of chicken Foxp3 will help to define avian regulatory T cells and to analyze their functional properties and thereby advance the field of avian immunology.

Název v anglickém jazyce

The Discovery of Chicken Foxp3 Demands Redefinition of Avian Regulatory T Cells

Popis výsledku anglicky

Since the publication of the first chicken genome sequence, we have encountered genes playing key roles in mammalian immunology, but being seemingly absent in birds. One of those was, until recently, Foxp3, the master transcription factor of regulatory T cells in mammals. Therefore, avian regulatory T cell research is still poorly standardized. In this study we identify a chicken ortholog of Foxp3. We prove sequence homology with known mammalian and sauropsid sequences, but also reveal differences in major domains. Expression profiling shows an association of Foxp3 and CD25 expression levels in CD4(+) CD25(+) peripheral T cells and identifies a CD4(-) CD25(+) Foxp3(high) subset of thymic lymphocytes that likely represents yet undescribed avian regulatory T precursor cells. We conclude that Foxp3 is existent in chickens and that it shares certain functional characteristics with its mammalian ortholog. Nevertheless, pathways for regulatory T cell development and Foxp3 function are likely to differ between mammals and birds. The identification and characterization of chicken Foxp3 will help to define avian regulatory T cells and to analyze their functional properties and thereby advance the field of avian immunology.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

30102 - Immunology

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Immunology

ISSN

0022-1767

e-ISSN

1550-6606

Svazek periodika

208

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

1128-1138

Kód UT WoS článku

000763497200003

EID výsledku v databázi Scopus

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