Beyond FoxP3-Identification of a Chicken Regulatory T Cell Signature

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F25%3A00645080" target="_blank" >RIV/68378050:_____/25:00645080 - isvavai.cz</a>

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/10.1002/eji.70106" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1002/eji.70106</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/eji.70106" target="_blank" >10.1002/eji.70106</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Beyond FoxP3-Identification of a Chicken Regulatory T Cell Signature

Popis výsledku v původním jazyce

Regulatory T cells (Tregs), defined by the lineage-specific transcription factor FoxP3, are crucial for immune regulation and have been studied extensively in mammals. However, avian Tregs remain poorly characterized, leaving gaps in our understanding of their evolutionary conservation and unique features. In this study, we investigated the phenotype of chicken Tregs to define reliable markers for their identification and characterization. We analyzed CD4(+) splenocytes sorted into CD25(negative), CD25(low), and CD25(high) subpopulations using RNA sequencing. FOXP3 and other Treg-associated genes were expressed in both CD25(low) and CD25(high) populations, showing that CD25 expression alone is insufficient to distinguish chicken Tregs. To refine the marker profile, we evaluated additional markers, including CTLA-4 and GITR. Notably, we describe for the first time a chicken-specific CTLA-4 antibody, which uniquely stains CTLA-4 exclusively in intracellular (ic) compartments, distinguishing it from mammalian counterparts. Single-cell RNA sequencing further confirmed distinct FOXP3(+) clusters enriched for expression of CTLA4 and TNFRSF18 (encoding GITR). While CTLA-4's ic expression limits usability in functional assays, the combination of CD4(+)/CD25(+)/CTLA-4(+)/GITR(+) represents the most accurate characterization of putative chicken Tregs to date. These findings highlight evolutionary conservation and species-specific differences in Treg markers, providing the foundation for future studies on chicken Treg functionality.

Název v anglickém jazyce

Beyond FoxP3-Identification of a Chicken Regulatory T Cell Signature

Popis výsledku anglicky

Regulatory T cells (Tregs), defined by the lineage-specific transcription factor FoxP3, are crucial for immune regulation and have been studied extensively in mammals. However, avian Tregs remain poorly characterized, leaving gaps in our understanding of their evolutionary conservation and unique features. In this study, we investigated the phenotype of chicken Tregs to define reliable markers for their identification and characterization. We analyzed CD4(+) splenocytes sorted into CD25(negative), CD25(low), and CD25(high) subpopulations using RNA sequencing. FOXP3 and other Treg-associated genes were expressed in both CD25(low) and CD25(high) populations, showing that CD25 expression alone is insufficient to distinguish chicken Tregs. To refine the marker profile, we evaluated additional markers, including CTLA-4 and GITR. Notably, we describe for the first time a chicken-specific CTLA-4 antibody, which uniquely stains CTLA-4 exclusively in intracellular (ic) compartments, distinguishing it from mammalian counterparts. Single-cell RNA sequencing further confirmed distinct FOXP3(+) clusters enriched for expression of CTLA4 and TNFRSF18 (encoding GITR). While CTLA-4's ic expression limits usability in functional assays, the combination of CD4(+)/CD25(+)/CTLA-4(+)/GITR(+) represents the most accurate characterization of putative chicken Tregs to date. These findings highlight evolutionary conservation and species-specific differences in Treg markers, providing the foundation for future studies on chicken Treg functionality.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30102 - Immunology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

European Journal of Immunology

ISSN

0014-2980

e-ISSN

1521-4141

Svazek periodika

55

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

13

Strana od-do

e70106

Kód UT WoS článku

001651771900018

EID výsledku v databázi Scopus

2-s2.0-105025378652