Dynamic interaction of spliceosomal snRNPs with coilin explains Cajal body characteristics

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F25%3A00644361" target="_blank" >RIV/68378050:_____/25:00644361 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/25:10515580

Výsledek na webu

<a href="https://rupress.org/jcb/article-abstract/224/8/e202309128/278087/Dynamic-interaction-of-spliceosomal-snRNPs-with?redirectedFrom=fulltext" target="_blank" >https://rupress.org/jcb/article-abstract/224/8/e202309128/278087/Dynamic-interaction-of-spliceosomal-snRNPs-with?redirectedFrom=fulltext</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1083/jcb.202309128" target="_blank" >10.1083/jcb.202309128</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Dynamic interaction of spliceosomal snRNPs with coilin explains Cajal body characteristics

Popis výsledku v původním jazyce

The Cajal body (CB) is a conserved non-membrane nuclear structure where several steps of small nuclear RNP particle (snRNP) biogenesis take place. It has been proposed that CB formation follows a liquid-liquid phase separation model, but this hypothesis has never been rigorously tested. Here, we applied live-cell imaging to show that the key CB assembly factor coilin is mobile within the CB, and we revealed a diffusion barrier that limits the coilin exchange between CBs and the nucleoplasm. We generated single aa mutations and demonstrated that RNA-dependent coilin oligomerization and coilin interaction with snRNP are essential for CB formation and maintenance. We applied these data to formulate a mathematical model that links the movement of coilin within the nucleoplasm, CB, and across the boundary with its oligomerization and snRNP binding. Our results illustrate CB as a structure dynamically responding to snRNP assembly and recycling.

Název v anglickém jazyce

Dynamic interaction of spliceosomal snRNPs with coilin explains Cajal body characteristics

Popis výsledku anglicky

The Cajal body (CB) is a conserved non-membrane nuclear structure where several steps of small nuclear RNP particle (snRNP) biogenesis take place. It has been proposed that CB formation follows a liquid-liquid phase separation model, but this hypothesis has never been rigorously tested. Here, we applied live-cell imaging to show that the key CB assembly factor coilin is mobile within the CB, and we revealed a diffusion barrier that limits the coilin exchange between CBs and the nucleoplasm. We generated single aa mutations and demonstrated that RNA-dependent coilin oligomerization and coilin interaction with snRNP are essential for CB formation and maintenance. We applied these data to formulate a mathematical model that links the movement of coilin within the nucleoplasm, CB, and across the boundary with its oligomerization and snRNP binding. Our results illustrate CB as a structure dynamically responding to snRNP assembly and recycling.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Cell Biology

ISSN

0021-9525

e-ISSN

1540-8140

Svazek periodika

224

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

29

Strana od-do

e202309128

Kód UT WoS článku

001516628300001

EID výsledku v databázi Scopus

2-s2.0-105009658082