Optimizing atomic force microscopy for characterization of diamond-protein interfaces

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F11%3A00375015" target="_blank" >RIV/68378271:_____/11:00375015 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1186/1556-276X-6-337" target="_blank" >http://dx.doi.org/10.1186/1556-276X-6-337</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/1556-276X-6-337" target="_blank" >10.1186/1556-276X-6-337</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Optimizing atomic force microscopy for characterization of diamond-protein interfaces

Popis výsledku v původním jazyce

Atomic force microscopy (AFM) in contact mode and tapping mode is employed for high resolution studies of soft organic molecules (fetal bovine serum proteins) on hard inorganic diamond substrates in solution and air. Various effects in morphology and phase measurements related to the cantilever spring constant, amplitude of tip oscillations, surface approach, tip shape and condition are demonstrated and discussed based on the proposed schematic models. We show that both diamond and proteins can be mechanically modified by Si AFM cantilever. We propose how to choose suitable cantilever type, optimize scanning parameters, recognize and minimize various artifacts, and obtain reliable AFM data both in solution and in air to reveal microscopic characteristics of protein-diamond interfaces. We also suggest that monocrystalline diamond is well defined substrate that can be applicable for fundamental studies of molecules on surfaces in general.

Název v anglickém jazyce

Optimizing atomic force microscopy for characterization of diamond-protein interfaces

Popis výsledku anglicky

Atomic force microscopy (AFM) in contact mode and tapping mode is employed for high resolution studies of soft organic molecules (fetal bovine serum proteins) on hard inorganic diamond substrates in solution and air. Various effects in morphology and phase measurements related to the cantilever spring constant, amplitude of tip oscillations, surface approach, tip shape and condition are demonstrated and discussed based on the proposed schematic models. We show that both diamond and proteins can be mechanically modified by Si AFM cantilever. We propose how to choose suitable cantilever type, optimize scanning parameters, recognize and minimize various artifacts, and obtain reliable AFM data both in solution and in air to reveal microscopic characteristics of protein-diamond interfaces. We also suggest that monocrystalline diamond is well defined substrate that can be applicable for fundamental studies of molecules on surfaces in general.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BM - Fyzika pevných látek a magnetismus

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nanoscale Research Letters

ISSN

1931-7573

e-ISSN

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Svazek periodika

6

Číslo periodika v rámci svazku

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Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

"337/1"-"337/10"

Kód UT WoS článku

000292289900005

EID výsledku v databázi Scopus

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