Charge transfer through DNA/DNA duplexes and DNA/RNA hybrids: complex theoretical and experimental studies

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F13%3A00396365" target="_blank" >RIV/68378271:_____/13:00396365 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/86652036:_____/13:00396365 RIV/61388963:_____/13:00396365 RIV/00216305:26310/13:PU104535

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.bpc.2013.07.009" target="_blank" >http://dx.doi.org/10.1016/j.bpc.2013.07.009</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bpc.2013.07.009" target="_blank" >10.1016/j.bpc.2013.07.009</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Charge transfer through DNA/DNA duplexes and DNA/RNA hybrids: complex theoretical and experimental studies

Popis výsledku v původním jazyce

Oligonucleotides conduct electric charge via various mechanisms and their characterization and understanding is a very important and complicated task. In this work, experimental (temperature dependent steady state fluorescence spectroscopy, time-resolvedfluorescence spectroscopy) and theoretical (Density Functional Theory) approaches were combined to study charge transfer processes in short DNA/DNA and RNA/DNA duplexes with virtually equivalent sequences. The experimental results were consistent with the theoretical model ? the delocalized nature of HOMO orbitals and holes, base stacking, electronic coupling and conformational flexibility formed the conditions for more effective short distance charge transfer processes in RNA/DNAhybrids. RNA/DNA and DNA/DNA charge transfer properties were strongly connectedwith temperature affected structural changes ofmolecular systems?charge transfer could be used as a probe of even tiny changes of molecular structures and settings.

Název v anglickém jazyce

Charge transfer through DNA/DNA duplexes and DNA/RNA hybrids: complex theoretical and experimental studies

Popis výsledku anglicky

Oligonucleotides conduct electric charge via various mechanisms and their characterization and understanding is a very important and complicated task. In this work, experimental (temperature dependent steady state fluorescence spectroscopy, time-resolvedfluorescence spectroscopy) and theoretical (Density Functional Theory) approaches were combined to study charge transfer processes in short DNA/DNA and RNA/DNA duplexes with virtually equivalent sequences. The experimental results were consistent with the theoretical model ? the delocalized nature of HOMO orbitals and holes, base stacking, electronic coupling and conformational flexibility formed the conditions for more effective short distance charge transfer processes in RNA/DNAhybrids. RNA/DNA and DNA/DNA charge transfer properties were strongly connectedwith temperature affected structural changes ofmolecular systems?charge transfer could be used as a probe of even tiny changes of molecular structures and settings.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

JJ - Ostatní materiály

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biophysical Chemistry

ISSN

0301-4622

e-ISSN

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Svazek periodika

180-181

Číslo periodika v rámci svazku

Oct-Nov

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

127-134

Kód UT WoS článku

000324898700015

EID výsledku v databázi Scopus

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