Design and synthesis of a novel trinuclear palladium(II) complex containing an oxime chelate ligand: determining the interaction mechanism with the DNA groove and BSA site I by spectroscopic and molecular dynamics simulation approaches

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F15%3A00456346" target="_blank" >RIV/68378271:_____/15:00456346 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1039/C5NJ01280E" target="_blank" >http://dx.doi.org/10.1039/C5NJ01280E</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/C5NJ01280E" target="_blank" >10.1039/C5NJ01280E</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Design and synthesis of a novel trinuclear palladium(II) complex containing an oxime chelate ligand: determining the interaction mechanism with the DNA groove and BSA site I by spectroscopic and molecular dynamics simulation approaches

Popis výsledku v původním jazyce

The novel trinuclear Pd(II) complex with an aryl oxime ligand, [Pd3(C,N-(C6H4C(Cl)QNO)-4)6], was synthesized and structurally characterized by elemental analysis (C, H, N), IR, resonance signals in the NMR, and single crystal X-ray crystallography. The interaction ability of the complex with native calf thymus DNA (CT-DNA) was monitored as a function of the metal complex?DNA molar ratio by UV-Vis absorption spectrophotometry, fluorescence spectroscopy, circular dichroism (CD) and thermal denaturation methods. All the experimental evidence indicated that this complex could strongly bind to CT-DN A via a groove mechanism. Further, the albumin interactions of the complex were investigated using fluorescence quenching and synchronous fluorescence spectra.The results of fluorescence titration suggested that the fluorescence quenching of BSA by the complex was a static quenching procedure.

Název v anglickém jazyce

Design and synthesis of a novel trinuclear palladium(II) complex containing an oxime chelate ligand: determining the interaction mechanism with the DNA groove and BSA site I by spectroscopic and molecular dynamics simulation approaches

Popis výsledku anglicky

The novel trinuclear Pd(II) complex with an aryl oxime ligand, [Pd3(C,N-(C6H4C(Cl)QNO)-4)6], was synthesized and structurally characterized by elemental analysis (C, H, N), IR, resonance signals in the NMR, and single crystal X-ray crystallography. The interaction ability of the complex with native calf thymus DNA (CT-DNA) was monitored as a function of the metal complex?DNA molar ratio by UV-Vis absorption spectrophotometry, fluorescence spectroscopy, circular dichroism (CD) and thermal denaturation methods. All the experimental evidence indicated that this complex could strongly bind to CT-DN A via a groove mechanism. Further, the albumin interactions of the complex were investigated using fluorescence quenching and synchronous fluorescence spectra.The results of fluorescence titration suggested that the fluorescence quenching of BSA by the complex was a static quenching procedure.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CC - Organická chemie

OECD FORD obor

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Projekt

<a href="/cs/project/GA14-03276S" target="_blank" >GA14-03276S: Nesférické efekty a jejich využití pro statisticky korektní analýzu krystalových struktur</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

New Journal of Chemistry

ISSN

1144-0546

e-ISSN

—

Svazek periodika

39

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

8708-8719

Kód UT WoS článku

000363668200059

EID výsledku v databázi Scopus

2-s2.0-84945971995