Dose-Rate effects in breaking DNA strands by short pulses of extreme ultraviolet radiation
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F18%3A00496971" target="_blank" >RIV/68378271:_____/18:00496971 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/61389021:_____/18:00503532 RIV/61389005:_____/18:00496971 RIV/68407700:21340/18:00324188 RIV/61388955:_____/18:00506556
Výsledek na webu
<a href="https://doi.org/10.1667/RR14825.1" target="_blank" >https://doi.org/10.1667/RR14825.1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1667/RR14825.1" target="_blank" >10.1667/RR14825.1</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Dose-Rate effects in breaking DNA strands by short pulses of extreme ultraviolet radiation
Popis výsledku v původním jazyce
Dose-rate effects on strand break formation in plasmid DNA induced by pulsed extreme ultraviolet (XUV) radiation were examined. Dose delivered to the target molecule was controlled by attenuating the incident photon flux using aluminum filters as well as by changing the DNA/buffer-salt ratio in the irradiated sample. Irradiated samples were examined using agarose gel electrophoresis. Yields of single- and double-strand breaks (SSBs and DSBs) were determined as a function of the incident photon fluence. Damaged DNA was inspected by means of atomic force microscopy (AFM). Both SSB and DSB yields decreased with dose rate increase. Quantum yields of SSBs at the highest photon fluence were comparable to yields of DSBs found after synchrotron irradiation. In conclusion, complex and/or clustered damages other than cross-links do not appear to be induced under the radiation conditions applied in this study.
Název v anglickém jazyce
Dose-Rate effects in breaking DNA strands by short pulses of extreme ultraviolet radiation
Popis výsledku anglicky
Dose-rate effects on strand break formation in plasmid DNA induced by pulsed extreme ultraviolet (XUV) radiation were examined. Dose delivered to the target molecule was controlled by attenuating the incident photon flux using aluminum filters as well as by changing the DNA/buffer-salt ratio in the irradiated sample. Irradiated samples were examined using agarose gel electrophoresis. Yields of single- and double-strand breaks (SSBs and DSBs) were determined as a function of the incident photon fluence. Damaged DNA was inspected by means of atomic force microscopy (AFM). Both SSB and DSB yields decreased with dose rate increase. Quantum yields of SSBs at the highest photon fluence were comparable to yields of DSBs found after synchrotron irradiation. In conclusion, complex and/or clustered damages other than cross-links do not appear to be induced under the radiation conditions applied in this study.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10306 - Optics (including laser optics and quantum optics)
Návaznosti výsledku
Projekt
<a href="/cs/project/GA13-28721S" target="_blank" >GA13-28721S: Účinky extrémního ultrafialového laserového záření na modelové biomembrány a povrch živých buněk</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2018
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Radiation Research
ISSN
0033-7587
e-ISSN
1938-5404
Svazek periodika
189
Číslo periodika v rámci svazku
5
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
11
Strana od-do
466-476
Kód UT WoS článku
000430572800003
EID výsledku v databázi Scopus
2-s2.0-85045934898