Breaking DNA strands by extreme-ultraviolet laser pulses in vacuum

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F15%3A00448547" target="_blank" >RIV/68378271:_____/15:00448547 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389005:_____/15:00448547 RIV/68407700:21340/15:00235107

Výsledek na webu

<a href="http://dx.doi.org/10.1103/PhysRevE.91.042718" target="_blank" >http://dx.doi.org/10.1103/PhysRevE.91.042718</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1103/PhysRevE.91.042718" target="_blank" >10.1103/PhysRevE.91.042718</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Breaking DNA strands by extreme-ultraviolet laser pulses in vacuum

Popis výsledku v původním jazyce

Ionizing radiation induces a variety of DNA damages including single-strand breaks (SSBs), double-strand breaks (DSBs), abasic sites, modified sugars, and bases. We have studied the nature of DNA damage induced directly by the pulsed 46.9nm (26.5 eV) radiation provided by an extreme ultraviolet (XUV) capillary-discharge Ne-like Ar laser (CDL). Doses up to 45 kGy were delivered with a repetition rate of 3 Hz. We studied the dependence of the yield of SSBs and DSBs of a simple model of DNA molecule (pBR322) on the CDL pulse fluence. Agarose gel electrophoresis method was used for determination of both SSB and DSB yields. The action cross sections of the single and double-strand breaks of pBR322 plasmid DNA in solid state were determined. We observed an increase in the efficiency of strand-break induction in the supercoiled DNA as a function of laser pulse fluence. Results are compared to those acquired at synchrotron radiation facilities and other sources of XUV and soft x-ray radiation.

Název v anglickém jazyce

Breaking DNA strands by extreme-ultraviolet laser pulses in vacuum

Popis výsledku anglicky

Ionizing radiation induces a variety of DNA damages including single-strand breaks (SSBs), double-strand breaks (DSBs), abasic sites, modified sugars, and bases. We have studied the nature of DNA damage induced directly by the pulsed 46.9nm (26.5 eV) radiation provided by an extreme ultraviolet (XUV) capillary-discharge Ne-like Ar laser (CDL). Doses up to 45 kGy were delivered with a repetition rate of 3 Hz. We studied the dependence of the yield of SSBs and DSBs of a simple model of DNA molecule (pBR322) on the CDL pulse fluence. Agarose gel electrophoresis method was used for determination of both SSB and DSB yields. The action cross sections of the single and double-strand breaks of pBR322 plasmid DNA in solid state were determined. We observed an increase in the efficiency of strand-break induction in the supercoiled DNA as a function of laser pulse fluence. Results are compared to those acquired at synchrotron radiation facilities and other sources of XUV and soft x-ray radiation.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Physical Review. E

ISSN

1539-3755

e-ISSN

—

Svazek periodika

91

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

"042718-1"-"042718-8"

Kód UT WoS článku

000353637500008

EID výsledku v databázi Scopus

2-s2.0-84929192771