Utilization of reactive oxygen species generated by electric discharge in biomedical engineering

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F18%3A00522122" target="_blank" >RIV/68378271:_____/18:00522122 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1615/plasmamed.2018024524" target="_blank" >https://doi.org/10.1615/plasmamed.2018024524</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1615/PlasmaMed.2018024524" target="_blank" >10.1615/PlasmaMed.2018024524</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Utilization of reactive oxygen species generated by electric discharge in biomedical engineering

Popis výsledku v původním jazyce

The discharge singlet oxygen generator-6 (DSOG-6) is a device generating reactive oxygen and nitrogen species (including singlet oxygen, ozone, atomic oxygen, NOx) by a hybrid RF/DC electric discharge. These reactive species are often used in biomedicine. In this study, reactions of selected cell cultures (B16, HT29, and HaCat) with DSOG-6-generated particles were observed and analyzed. Cell cultures were exposed to singlet oxygen or reactive oxygen species for different time periodes. After the exposure, a microscopic analysis of the cytomorphology of cells was performed. Furthermore, cell proliferation of the B16 cell line was investigated by the xCELLigence System (Acea Biosciences Inc., San Diego, CA). Finally, preliminary results of qPCR from B16 exposed to singlet oxygen are presented. The main goal of this study was to evaluate if DSOG-6 could be used in oncology and dermatology. The secondary aim was to observe the dependence of cell lines’ behavior on exposure parameters.n

Název v anglickém jazyce

Utilization of reactive oxygen species generated by electric discharge in biomedical engineering

Popis výsledku anglicky

The discharge singlet oxygen generator-6 (DSOG-6) is a device generating reactive oxygen and nitrogen species (including singlet oxygen, ozone, atomic oxygen, NOx) by a hybrid RF/DC electric discharge. These reactive species are often used in biomedicine. In this study, reactions of selected cell cultures (B16, HT29, and HaCat) with DSOG-6-generated particles were observed and analyzed. Cell cultures were exposed to singlet oxygen or reactive oxygen species for different time periodes. After the exposure, a microscopic analysis of the cytomorphology of cells was performed. Furthermore, cell proliferation of the B16 cell line was investigated by the xCELLigence System (Acea Biosciences Inc., San Diego, CA). Finally, preliminary results of qPCR from B16 exposed to singlet oxygen are presented. The main goal of this study was to evaluate if DSOG-6 could be used in oncology and dermatology. The secondary aim was to observe the dependence of cell lines’ behavior on exposure parameters.n

Druh

J_ost - Ostatní články v recenzovaných periodicích

CEP obor

—

OECD FORD obor

10305 - Fluids and plasma physics (including surface physics)

Projekt

<a href="/cs/project/GAP102%2F12%2F0723" target="_blank" >GAP102/12/0723: Hybridní radiofrekvenční/obloukový generátor singletového kyslíku</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plasma Medicine

ISSN

1947-5764

e-ISSN

—

Svazek periodika

8

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

147-154

Kód UT WoS článku

—

EID výsledku v databázi Scopus

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