Utilisation of reactive oxygen species generated by electric discharge in biomedical engineering

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F18%3A00317627" target="_blank" >RIV/68407700:21460/18:00317627 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.dl.begellhouse.com/journals/5a5b4a3d419387fb,530c9c0e78fa67b7,60fa5b987ee94cb2.html" target="_blank" >http://www.dl.begellhouse.com/journals/5a5b4a3d419387fb,530c9c0e78fa67b7,60fa5b987ee94cb2.html</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1615/PlasmaMed.2018024524" target="_blank" >10.1615/PlasmaMed.2018024524</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Utilisation of reactive oxygen species generated by electric discharge in biomedical engineering

Popis výsledku v původním jazyce

Discharge Singlet Oxygen Generator 6 (DSOG-6) is a device generating reactive oxygen and nitrogen species (including singlet oxygen, ozone, atomic oxygen, NOx) by a hybrid RF/DC electric discharge. These reactive species are often used in biomedicine. In this work reactions of selected cell cultures (B16, HT29 and HaCat) with DSOG-6-generated particles were observed and analyzed. Cell cultures were exposed to singlet oxygen or reactive oxygen species generated by a hybrid electric discharge for different time periods. After the exposure a microscopic analysis of the cytomorphology of cells was carried out. Furthermore, cell proliferation of B16 cell line was investigated by xCELLigence System. Finally, preliminary results of qPCR from B16 exposed to singlet oxygen are presented. The main goal of this work was to evaluate if singlet oxygen/reactive oxygen species generated by DSOG-6 could be used in biomedical application (concretely in oncology and dermatology) and DSOG-6 could become a new biomedical device. The secondary aim was to observe the dependence of cell lines behavior on exposure parameters (time intervals, particles in discharge, etc.).

Název v anglickém jazyce

Utilisation of reactive oxygen species generated by electric discharge in biomedical engineering

Popis výsledku anglicky

Discharge Singlet Oxygen Generator 6 (DSOG-6) is a device generating reactive oxygen and nitrogen species (including singlet oxygen, ozone, atomic oxygen, NOx) by a hybrid RF/DC electric discharge. These reactive species are often used in biomedicine. In this work reactions of selected cell cultures (B16, HT29 and HaCat) with DSOG-6-generated particles were observed and analyzed. Cell cultures were exposed to singlet oxygen or reactive oxygen species generated by a hybrid electric discharge for different time periods. After the exposure a microscopic analysis of the cytomorphology of cells was carried out. Furthermore, cell proliferation of B16 cell line was investigated by xCELLigence System. Finally, preliminary results of qPCR from B16 exposed to singlet oxygen are presented. The main goal of this work was to evaluate if singlet oxygen/reactive oxygen species generated by DSOG-6 could be used in biomedical application (concretely in oncology and dermatology) and DSOG-6 could become a new biomedical device. The secondary aim was to observe the dependence of cell lines behavior on exposure parameters (time intervals, particles in discharge, etc.).

Druh

J_ost - Ostatní články v recenzovaných periodicích

CEP obor

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OECD FORD obor

10305 - Fluids and plasma physics (including surface physics)

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plasma Medicine

ISSN

1947-5772

e-ISSN

1947-5772

Svazek periodika

8

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

147-154

Kód UT WoS článku

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EID výsledku v databázi Scopus

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