Infrared absorption spectroscopy of albumin binding with amine-containing plasma polymer coatings on nanoporous diamond surfaces

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F19%3A00517868" target="_blank" >RIV/68378271:_____/19:00517868 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68407700:21230/19:00335376

Výsledek na webu

<a href="https://doi.org/10.1021/acs.langmuir.9b02327" target="_blank" >https://doi.org/10.1021/acs.langmuir.9b02327</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acs.langmuir.9b02327" target="_blank" >10.1021/acs.langmuir.9b02327</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Infrared absorption spectroscopy of albumin binding with amine-containing plasma polymer coatings on nanoporous diamond surfaces

Popis výsledku v původním jazyce

Nanocrystalline diamond (NCD) layers functionalized with amine-containing functional groups have generated considerable interest as biocompatible substrates for attachment of biomolecules and cells. Here we prepare nanoporous diamond layers with the surfaces modified by H, O plasma, and conformal 7 nm amine-containing plasma polymer (PP). Immobilization of bovine serum albumin (BSA) molecules is characterized on such surfaces. GAR-FTIR as well as XPS show that concentration of amine-containing bonds after BSA exposure depends on the type of NCD surface modification. AFM measurements reveal that BSA proteins are physisorbed on H- and O-terminated diamond surfaces in different thicknesses and morphology. When the diamond layers are coated with the amine-containing PP, BSA molecules assume similar thickness and morphology, and their adhesion is significantly increased on both types of the diamond surfaces.n

Název v anglickém jazyce

Infrared absorption spectroscopy of albumin binding with amine-containing plasma polymer coatings on nanoporous diamond surfaces

Popis výsledku anglicky

Nanocrystalline diamond (NCD) layers functionalized with amine-containing functional groups have generated considerable interest as biocompatible substrates for attachment of biomolecules and cells. Here we prepare nanoporous diamond layers with the surfaces modified by H, O plasma, and conformal 7 nm amine-containing plasma polymer (PP). Immobilization of bovine serum albumin (BSA) molecules is characterized on such surfaces. GAR-FTIR as well as XPS show that concentration of amine-containing bonds after BSA exposure depends on the type of NCD surface modification. AFM measurements reveal that BSA proteins are physisorbed on H- and O-terminated diamond surfaces in different thicknesses and morphology. When the diamond layers are coated with the amine-containing PP, BSA molecules assume similar thickness and morphology, and their adhesion is significantly increased on both types of the diamond surfaces.n

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10610 - Biophysics

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Langmuir

ISSN

0743-7463

e-ISSN

—

Svazek periodika

35

Číslo periodika v rámci svazku

43

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

13844-13852

Kód UT WoS článku

000493586900006

EID výsledku v databázi Scopus

2-s2.0-85073826868