Diamond aminated with plasma polymer as biocompatible substrate for protein attachment

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F19%3A00521839" target="_blank" >RIV/68378271:_____/19:00521839 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Diamond aminated with plasma polymer as biocompatible substrate for protein attachment

Popis výsledku v původním jazyce

Nanocrystalline diamond (NCD) films are known as a highly attractive material for biosensing applications due to their unique semiconducting properties. In this study, we investigate the immobilization of bovine serum albumin (BSA) molecules on the surface of flat and nanoporous diamond films terminated by hydrogen, oxygen or amine-containing plasma polymer (thickness 7 nm). GAR-FTIR and XPS measurements confirmed that the concentration of amine-containing bonds after the BSA attachment depends on the NCD surface termination. We found that the amine-containing polymer provides a strong interlink between BSA protein and the NCD film while preserving its globular conformation and probably also its biochemical functionality. This work was supported by Operational Programme Research, Development and Education financed by European Structural and Investment Funds and the Czech Ministry of Education, Youth and Sports (Project No. SOLID21 - CZ.02.1.01/0.0/0.0/16_019/0000760).n

Název v anglickém jazyce

Diamond aminated with plasma polymer as biocompatible substrate for protein attachment

Popis výsledku anglicky

Nanocrystalline diamond (NCD) films are known as a highly attractive material for biosensing applications due to their unique semiconducting properties. In this study, we investigate the immobilization of bovine serum albumin (BSA) molecules on the surface of flat and nanoporous diamond films terminated by hydrogen, oxygen or amine-containing plasma polymer (thickness 7 nm). GAR-FTIR and XPS measurements confirmed that the concentration of amine-containing bonds after the BSA attachment depends on the NCD surface termination. We found that the amine-containing polymer provides a strong interlink between BSA protein and the NCD film while preserving its globular conformation and probably also its biochemical functionality. This work was supported by Operational Programme Research, Development and Education financed by European Structural and Investment Funds and the Czech Ministry of Education, Youth and Sports (Project No. SOLID21 - CZ.02.1.01/0.0/0.0/16_019/0000760).n

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10610 - Biophysics

Projekt

<a href="/cs/project/EF16_019%2F0000760" target="_blank" >EF16_019/0000760: Fyzika pevných látek pro 21. století</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů