Microfluidic diamond biosensor using NV centre charge state detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F19%3A00518038" target="_blank" >RIV/68378271:_____/19:00518038 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68407700:21460/19:00328300

Výsledek na webu

<a href="http://dx.doi.org/10.1007/978-981-10-9023-3_6" target="_blank" >http://dx.doi.org/10.1007/978-981-10-9023-3_6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-981-10-9023-3_6" target="_blank" >10.1007/978-981-10-9023-3_6</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Microfluidic diamond biosensor using NV centre charge state detection

Popis výsledku v původním jazyce

We develop DNA sensors based on charge switching in colour centres in diamond. The presented method allows the combination of luminescence sensor and electrochemical sensor working on the principle of electrochemical impedance spectroscopy (EIS). The structure consists of highly boron doped diamond electrode on which an intrinsic diamond is deposited. This intrinsic layer contains NV colour centres. The device is then embedded in PDMS microfluidic flow cell and covered by a transparent ITO electrode. The switching of the NV centre charge state as a response, on diamond surface termination, is crucial tool for the sensitive charged molecules sensing. We demonstrated high sensitivity of the near surface NV centres on surface charge termination. O- terminated surface results in a preferable NV centre charge state of NV0 or NV−, whereas H- terminated surface leads to non-PL NV+ charge state. By this principle any charged molecule can be detected by a customized surface functionalization.

Název v anglickém jazyce

Microfluidic diamond biosensor using NV centre charge state detection

Popis výsledku anglicky

We develop DNA sensors based on charge switching in colour centres in diamond. The presented method allows the combination of luminescence sensor and electrochemical sensor working on the principle of electrochemical impedance spectroscopy (EIS). The structure consists of highly boron doped diamond electrode on which an intrinsic diamond is deposited. This intrinsic layer contains NV colour centres. The device is then embedded in PDMS microfluidic flow cell and covered by a transparent ITO electrode. The switching of the NV centre charge state as a response, on diamond surface termination, is crucial tool for the sensitive charged molecules sensing. We demonstrated high sensitivity of the near surface NV centres on surface charge termination. O- terminated surface results in a preferable NV centre charge state of NV0 or NV−, whereas H- terminated surface leads to non-PL NV+ charge state. By this principle any charged molecule can be detected by a customized surface functionalization.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

10302 - Condensed matter physics (including formerly solid state physics, supercond.)

Projekt

<a href="/cs/project/GA16-16336S" target="_blank" >GA16-16336S: Kvantové detekční techologie: nanodiamanty pro sensing ve vnitrobuněčném prostředí</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

World Congress on Medical Physics and Biomedical Engineering 2018

ISBN

978-981-10-9022-6

ISSN

1680-0737

e-ISSN

—

Počet stran výsledku

5

Strana od-do

27-31

Název nakladatele

Springer

Místo vydání

Singapore

Místo konání akce

Prague

Datum konání akce

3. 6. 2018

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000449744300006