Electrospray sample injection for single-particle imaging with x-ray lasers
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F19%3A00521634" target="_blank" >RIV/68378271:_____/19:00521634 - isvavai.cz</a>
Výsledek na webu
<a href="http://hdl.handle.net/11104/0306226" target="_blank" >http://hdl.handle.net/11104/0306226</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1126/sciadv.aav8801" target="_blank" >10.1126/sciadv.aav8801</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Electrospray sample injection for single-particle imaging with x-ray lasers
Popis výsledku v původním jazyce
The possibility of imaging single proteins constitutes an exciting challenge for x-ray lasers. Despite encouraging results on large particles, imaging small particles has proven to be difficult for two reasons: not quite high enough pulse intensity from currently available x-ray lasers and, as we demonstrate here, contamination of the aerosolized molecules by nonvolatile contaminants in the solution. The amount of contamination on the sample depends on the initial droplet size during aerosolization. Here, we show that, with our electrospray injector, we can decrease the size of aerosol droplets and demonstrate virtually contaminant-free sample delivery of organelles, small virions, and proteins. The results presented here, together with the increased performance of next-generation x-ray lasers, constitute an important stepping stone toward the ultimate goal of protein structure determination from imaging at room temperature and high temporal resolution.
Název v anglickém jazyce
Electrospray sample injection for single-particle imaging with x-ray lasers
Popis výsledku anglicky
The possibility of imaging single proteins constitutes an exciting challenge for x-ray lasers. Despite encouraging results on large particles, imaging small particles has proven to be difficult for two reasons: not quite high enough pulse intensity from currently available x-ray lasers and, as we demonstrate here, contamination of the aerosolized molecules by nonvolatile contaminants in the solution. The amount of contamination on the sample depends on the initial droplet size during aerosolization. Here, we show that, with our electrospray injector, we can decrease the size of aerosol droplets and demonstrate virtually contaminant-free sample delivery of organelles, small virions, and proteins. The results presented here, together with the increased performance of next-generation x-ray lasers, constitute an important stepping stone toward the ultimate goal of protein structure determination from imaging at room temperature and high temporal resolution.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10305 - Fluids and plasma physics (including surface physics)
Návaznosti výsledku
Projekt
<a href="/cs/project/LQ1606" target="_blank" >LQ1606: ELI Beamlines: Mezinárodní centrum Excelemce</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2019
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Science Advances
ISSN
2375-2548
e-ISSN
—
Svazek periodika
5
Číslo periodika v rámci svazku
5
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
9
Strana od-do
1-9
Kód UT WoS článku
000470125000080
EID výsledku v databázi Scopus
2-s2.0-85065606358