Nanocrystalline diamond electrolyte-gates in field effect transistor for a prolific aptasensing HIV-1 tat on hydrogen-terminated surface

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F20%3A00538344" target="_blank" >RIV/68378271:_____/20:00538344 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68407700:21230/20:00342536

Výsledek na webu

<a href="https://ijneam.unimap.edu.my/images/PDF/IJNEAM%20APR%202020/Vol_13_No_2_2020_7_295-306.pdf" target="_blank" >https://ijneam.unimap.edu.my/images/PDF/IJNEAM%20APR%202020/Vol_13_No_2_2020_7_295-306.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Nanocrystalline diamond electrolyte-gates in field effect transistor for a prolific aptasensing HIV-1 tat on hydrogen-terminated surface

Popis výsledku v původním jazyce

This research investigated on Trans-activator of transcription (Tat) protein, a powerful viral gene activator that plays a pivotal role in the primary stage of the human immunodeficiency virus type 1 (HIV-1) replication. Dose-dependent interactions of HIV-1 Tat on NCD-EGFET-based RNA aptamer sensing surface were monitored and attained the detection down to 10 fM. The linear regression curve with 3σ estimation professed the sensitivity range to be 31.213 mV/log10 [Tat Concentration]M and the limit of detection of 6.18 fM. The selectivity analysis of NCD-EGFET was conducted with different proteins from HIV (Nef and p24) and Bovine Serum Albumin. Furthermore, to practice in the clinical application, HIV-1 Tat was spiked into the human blood serum and it displayed the genuine non-fouling interaction with the aptamer. The attained high-performance signal enhancement with nanocrystalline diamond-biosensing aids to circumvent the issues in the current diagnosis.

Název v anglickém jazyce

Nanocrystalline diamond electrolyte-gates in field effect transistor for a prolific aptasensing HIV-1 tat on hydrogen-terminated surface

Popis výsledku anglicky

This research investigated on Trans-activator of transcription (Tat) protein, a powerful viral gene activator that plays a pivotal role in the primary stage of the human immunodeficiency virus type 1 (HIV-1) replication. Dose-dependent interactions of HIV-1 Tat on NCD-EGFET-based RNA aptamer sensing surface were monitored and attained the detection down to 10 fM. The linear regression curve with 3σ estimation professed the sensitivity range to be 31.213 mV/log10 [Tat Concentration]M and the limit of detection of 6.18 fM. The selectivity analysis of NCD-EGFET was conducted with different proteins from HIV (Nef and p24) and Bovine Serum Albumin. Furthermore, to practice in the clinical application, HIV-1 Tat was spiked into the human blood serum and it displayed the genuine non-fouling interaction with the aptamer. The attained high-performance signal enhancement with nanocrystalline diamond-biosensing aids to circumvent the issues in the current diagnosis.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

21001 - Nano-materials (production and properties)

Projekt

<a href="/cs/project/EF16_019%2F0000760" target="_blank" >EF16_019/0000760: Fyzika pevných látek pro 21. století</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Nanoelectronics and Materials

ISSN

1985-5761

e-ISSN

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Svazek periodika

13

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

MY - Malajsie

Počet stran výsledku

12

Strana od-do

295-306

Kód UT WoS článku

000531045800006

EID výsledku v databázi Scopus

2-s2.0-85085315022