Can classical surface plasmon resonance advance via the coupling to other analytical approaches?

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F22%3A00566272" target="_blank" >RIV/68378271:_____/22:00566272 - isvavai.cz</a>

Výsledek na webu

<a href="https://hdl.handle.net/11104/0339377" target="_blank" >https://hdl.handle.net/11104/0339377</a>

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Can classical surface plasmon resonance advance via the coupling to other analytical approaches?

Popis výsledku v původním jazyce

For nearly 40 years, surface plasmon resonance (SPR) analysis has been used to better understand the binding interaction strength between surface immobilized bioreceptors and the analytes of interest. The advantage of surface plasmon resonance, over other affinity sensing approaches such as Western blots and ELISAs approaches, resides in its possibility to reveal binding kinetics in a label-free manner. The concept of surface plasmon resonance has in addition been widely employed for the development of biosensors capitalizing on its direct assay format, short response times, simple sample treatments along with multiplexed sensing possibilities. To this must be added the possibility to reach high sensitivity due to the capability of surface plasmon resonance to detect very small changes in refractive index at the sensing interfaces in particular for analytes of larger size such as cells (e.g., bacteria), proteins, peptides and oligonucleotides.

Název v anglickém jazyce

Can classical surface plasmon resonance advance via the coupling to other analytical approaches?

Popis výsledku anglicky

For nearly 40 years, surface plasmon resonance (SPR) analysis has been used to better understand the binding interaction strength between surface immobilized bioreceptors and the analytes of interest. The advantage of surface plasmon resonance, over other affinity sensing approaches such as Western blots and ELISAs approaches, resides in its possibility to reveal binding kinetics in a label-free manner. The concept of surface plasmon resonance has in addition been widely employed for the development of biosensors capitalizing on its direct assay format, short response times, simple sample treatments along with multiplexed sensing possibilities. To this must be added the possibility to reach high sensitivity due to the capability of surface plasmon resonance to detect very small changes in refractive index at the sensing interfaces in particular for analytes of larger size such as cells (e.g., bacteria), proteins, peptides and oligonucleotides.

Druh

O - Ostatní výsledky

CEP obor

—

OECD FORD obor

10610 - Biophysics

Projekt

<a href="/cs/project/GC22-30456J" target="_blank" >GC22-30456J: Plazmonický biosenzor pro kontinuální monitoring analytů s nízkou molekulární hmotností</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů