Iron oxide nanoparticles trigger endoplasmic reticulum damage in steatotic hepatic cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F23%3A00574215" target="_blank" >RIV/68378271:_____/23:00574215 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00023001:_____/23:00084083

Výsledek na webu

<a href="https://hdl.handle.net/11104/0344559" target="_blank" >https://hdl.handle.net/11104/0344559</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/D3NA00071K" target="_blank" >10.1039/D3NA00071K</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Iron oxide nanoparticles trigger endoplasmic reticulum damage in steatotic hepatic cells

Popis výsledku v původním jazyce

Iron oxide nanoparticles (IONPs) are being actively researched in various biomedical applications, particularly as magnetic resonance imaging contrast agents for diagnosing various liver pathologies. Emerging evidence suggests that IONPs may exacerbate hepatic steatosis and liver injury in susceptible livers. However, our understanding of how IONPs may affect steatotic cells at the sub-cellular level is still fragmented. In this study, we demonstrate that IONPs, at a dose that does not cause general toxicity in hepatic cells, induce significant toxicity in steatotic cells. Mechanistically, co-treatment with palmitic acid and IONPs resulted in endoplasmic reticulum (ER) stress, accompanied by the release of cathepsin B from lysosomes to the cytosol. The release of cathepsin B, along with ER stress, led to the activation of apoptotic cell death. This study provides important basic knowledge for the future optimization of IONPs as MRI contrast agents for various biomedical applications.

Název v anglickém jazyce

Iron oxide nanoparticles trigger endoplasmic reticulum damage in steatotic hepatic cells

Popis výsledku anglicky

Iron oxide nanoparticles (IONPs) are being actively researched in various biomedical applications, particularly as magnetic resonance imaging contrast agents for diagnosing various liver pathologies. Emerging evidence suggests that IONPs may exacerbate hepatic steatosis and liver injury in susceptible livers. However, our understanding of how IONPs may affect steatotic cells at the sub-cellular level is still fragmented. In this study, we demonstrate that IONPs, at a dose that does not cause general toxicity in hepatic cells, induce significant toxicity in steatotic cells. Mechanistically, co-treatment with palmitic acid and IONPs resulted in endoplasmic reticulum (ER) stress, accompanied by the release of cathepsin B from lysosomes to the cytosol. The release of cathepsin B, along with ER stress, led to the activation of apoptotic cell death. This study provides important basic knowledge for the future optimization of IONPs as MRI contrast agents for various biomedical applications.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10610 - Biophysics

Projekt

<a href="/cs/project/EF16_019%2F0000760" target="_blank" >EF16_019/0000760: Fyzika pevných látek pro 21. století</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nanoscale Advances

ISSN

2516-0230

e-ISSN

2516-0230

Svazek periodika

5

Číslo periodika v rámci svazku

16

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

19

Strana od-do

4250-4268

Kód UT WoS článku

001037449000001

EID výsledku v databázi Scopus

2-s2.0-85167418563