Osteoblasts win the race for the surface on DNA polyelectrolyte multilayer coatings against S. epidermidis but not against S. aureus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F25%3A00618663" target="_blank" >RIV/68378271:_____/25:00618663 - isvavai.cz</a>

Výsledek na webu

<a href="https://hdl.handle.net/11104/0365514" target="_blank" >https://hdl.handle.net/11104/0365514</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.colsurfb.2024.114336" target="_blank" >10.1016/j.colsurfb.2024.114336</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Osteoblasts win the race for the surface on DNA polyelectrolyte multilayer coatings against S. epidermidis but not against S. aureus

Popis výsledku v původním jazyce

Co-culture of staphylococcus (S. epidermidis and S. aureus) and SaOS-2 osteosarcoma cells was studied on chitosan-DNA polyelectrolyte multilayer coated glass based on the concept of “the race for the surface.” Staphylococcus was first deposited onto the surface in a microfluidic chamber to mimic peri-operative contamination, SaOS-2 cells were seeded. Both were cultured together on the surfaces for 24 h under flow. The presence of S. epidermidis decreased SaOS-2 cell number on all surfaces after 24 h. The cells that adhered spread equally well in the presence of low virulent S. epidermidis. Highly virulent S. aureus induced cell death of all adherent SaOS-2 cells on chitosan-DNA multilayer coated glass, a worse outcome than on uncoated glass. The outcome of our study demonstrates the need for in vitro co-culture assays to meaningfully bridge the gap in lab testing of biomaterials and their clinical evaluations where bacterial infection can occur.

Název v anglickém jazyce

Osteoblasts win the race for the surface on DNA polyelectrolyte multilayer coatings against S. epidermidis but not against S. aureus

Popis výsledku anglicky

Co-culture of staphylococcus (S. epidermidis and S. aureus) and SaOS-2 osteosarcoma cells was studied on chitosan-DNA polyelectrolyte multilayer coated glass based on the concept of “the race for the surface.” Staphylococcus was first deposited onto the surface in a microfluidic chamber to mimic peri-operative contamination, SaOS-2 cells were seeded. Both were cultured together on the surfaces for 24 h under flow. The presence of S. epidermidis decreased SaOS-2 cell number on all surfaces after 24 h. The cells that adhered spread equally well in the presence of low virulent S. epidermidis. Highly virulent S. aureus induced cell death of all adherent SaOS-2 cells on chitosan-DNA multilayer coated glass, a worse outcome than on uncoated glass. The outcome of our study demonstrates the need for in vitro co-culture assays to meaningfully bridge the gap in lab testing of biomaterials and their clinical evaluations where bacterial infection can occur.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10610 - Biophysics

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Colloids and Surfaces B-Biointerfaces

ISSN

0927-7765

e-ISSN

1873-4367

Svazek periodika

245

Číslo periodika v rámci svazku

Jan

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

114336

Kód UT WoS článku

001348558800001

EID výsledku v databázi Scopus

2-s2.0-85207651373