Copperfluidics: Controlled Cu0-Mediated SI-ATRP under Flow for High-Precision Polymer Brush Synthesis Monitored by QCM-D

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F25%3A00639780" target="_blank" >RIV/68378271:_____/25:00639780 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1109/IWASI66786.2025.11121930" target="_blank" >http://dx.doi.org/10.1109/IWASI66786.2025.11121930</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1109/IWASI66786.2025.11121930" target="_blank" >10.1109/IWASI66786.2025.11121930</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Copperfluidics: Controlled Cu0-Mediated SI-ATRP under Flow for High-Precision Polymer Brush Synthesis Monitored by QCM-D

Popis výsledku v původním jazyce

The development of robust antifouling surfaces is critical for reliable label-free biosensing in complex biological samples. One effective approach uses zwitterionic polymer brushes synthesized via surface-initiated atom transfer radical polymerization (SI-ATRP): a method where the CuI/CuII ratio can control the polymer brush characteristics, albeit where deoxygenated conditions complicate synthesis. Here, we use a copper-based microreactor to simultaneously deoxygenate the solution and generate CuI. Integration into a microfluidic system allowed controlled polymerization of an antifouling zwitterionic homopolymer brush, poly(carboxybetaine methacrylamide) (pCBMAA). The polymerization process is monitored in real time by the quartz crystal microbalance with dissipation monitoring (QCM-D) method. The resulting surfaces were characterized by FT-IR and spectroscopic ellipsometry, and the results were compared with those obtained by QCM-D method.The pCBMAA-coated surfaces exhibited excellent antifouling performance in 50% human blood plasma, confirming their suitability for biosensing in complex matrices. Furthermore, successful antibody functionalization enabled the detection of E. coli O157:H7, demonstrating the versatility of these brushes for specific biomolecular interactions.

Název v anglickém jazyce

Copperfluidics: Controlled Cu0-Mediated SI-ATRP under Flow for High-Precision Polymer Brush Synthesis Monitored by QCM-D

Popis výsledku anglicky

The development of robust antifouling surfaces is critical for reliable label-free biosensing in complex biological samples. One effective approach uses zwitterionic polymer brushes synthesized via surface-initiated atom transfer radical polymerization (SI-ATRP): a method where the CuI/CuII ratio can control the polymer brush characteristics, albeit where deoxygenated conditions complicate synthesis. Here, we use a copper-based microreactor to simultaneously deoxygenate the solution and generate CuI. Integration into a microfluidic system allowed controlled polymerization of an antifouling zwitterionic homopolymer brush, poly(carboxybetaine methacrylamide) (pCBMAA). The polymerization process is monitored in real time by the quartz crystal microbalance with dissipation monitoring (QCM-D) method. The resulting surfaces were characterized by FT-IR and spectroscopic ellipsometry, and the results were compared with those obtained by QCM-D method.The pCBMAA-coated surfaces exhibited excellent antifouling performance in 50% human blood plasma, confirming their suitability for biosensing in complex matrices. Furthermore, successful antibody functionalization enabled the detection of E. coli O157:H7, demonstrating the versatility of these brushes for specific biomolecular interactions.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

10404 - Polymer science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

2025 IEEE International Workshop on Advances in Sensors and Interfaces (IWASI)

ISBN

979-8-3315-6579-4

ISSN

2836-8681

e-ISSN

—

Počet stran výsledku

5

Strana od-do

11121930

Název nakladatele

IEEE

Místo vydání

Piscataway

Místo konání akce

Manfredonia

Datum konání akce

3. 7. 2025

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

001568944200029