Polylactide nanofibers with hydroxyapatite as growth substrates for osteoblast-like cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21220%2F14%3A00316032" target="_blank" >RIV/68407700:21220/14:00316032 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378271:_____/14:00434268 RIV/67985823:_____/14:00434268 RIV/61389013:_____/14:00434268 RIV/67985891:_____/14:00434268

Výsledek na webu

<a href="http://dx.doi.org/10.1002/jbm.a.35061" target="_blank" >http://dx.doi.org/10.1002/jbm.a.35061</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jbm.a.35061" target="_blank" >10.1002/jbm.a.35061</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Polylactide nanofibers with hydroxyapatite as growth substrates for osteoblast-like cells

Popis výsledku v původním jazyce

Various types of nanofibers are increasingly used in tissue engineering, mainly for their ability to mimic the architecture of tissue at the nanoscale. We evaluated the adhesion, growth, viability, and differentiation of human osteoblast-like MG 63 cells on polylactide (PLA) nanofibers prepared by needle-less electrospinning and loaded with 5 or 15 wt % of hydroxyapatite (HA) nanoparticles. On day 7 after seeding, the cell number was the highest on samples with 15 wt % of HA. This result was confirmed by the XTT test, especially after dynamic cultivation, when the number of metabolically active cells on these samples was even higher than on control polystyrene. Staining with a live/dead kit showed that the viability of cells on all nanofibrous scaffolds was very high and comparable to that on control polystyrene dishes. An enzyme-linked immunosorbent assay revealed that the concentration of osteocalcin was also higher in cells on samples with 15 wt % of HA. There was no immune activation of cells (measured by production of TNF-alpha), associated with the incorporation of HA. Moreover, the addition of HA suppressed the creep behavior of the scaffolds in their dry state. Thus, nanofibrous PLA scaffolds have potential for bone tissue engineering, particularly those with 15 wt % of HA. (C) 2013 Wiley Periodicals, Inc.

Název v anglickém jazyce

Polylactide nanofibers with hydroxyapatite as growth substrates for osteoblast-like cells

Popis výsledku anglicky

Various types of nanofibers are increasingly used in tissue engineering, mainly for their ability to mimic the architecture of tissue at the nanoscale. We evaluated the adhesion, growth, viability, and differentiation of human osteoblast-like MG 63 cells on polylactide (PLA) nanofibers prepared by needle-less electrospinning and loaded with 5 or 15 wt % of hydroxyapatite (HA) nanoparticles. On day 7 after seeding, the cell number was the highest on samples with 15 wt % of HA. This result was confirmed by the XTT test, especially after dynamic cultivation, when the number of metabolically active cells on these samples was even higher than on control polystyrene. Staining with a live/dead kit showed that the viability of cells on all nanofibrous scaffolds was very high and comparable to that on control polystyrene dishes. An enzyme-linked immunosorbent assay revealed that the concentration of osteocalcin was also higher in cells on samples with 15 wt % of HA. There was no immune activation of cells (measured by production of TNF-alpha), associated with the incorporation of HA. Moreover, the addition of HA suppressed the creep behavior of the scaffolds in their dry state. Thus, nanofibrous PLA scaffolds have potential for bone tissue engineering, particularly those with 15 wt % of HA. (C) 2013 Wiley Periodicals, Inc.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

21001 - Nano-materials (production and properties)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Biomedical Materials Research Part A

ISSN

1549-3296

e-ISSN

1552-4965

Svazek periodika

2014

Číslo periodika v rámci svazku

102/11

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

13

Strana od-do

3918-3930

Kód UT WoS článku

000343010100016

EID výsledku v databázi Scopus

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