The effect of polymeric membrane surface on HaCaT cell properties

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21220%2F17%3A00312513" target="_blank" >RIV/68407700:21220/17:00312513 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.micron.2017.07.006" target="_blank" >https://doi.org/10.1016/j.micron.2017.07.006</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.micron.2017.07.006" target="_blank" >10.1016/j.micron.2017.07.006</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The effect of polymeric membrane surface on HaCaT cell properties

Popis výsledku v původním jazyce

The control of the surface properties is an important issue for applicability of polymer membranes interacting with cells. In this work, the influence of surface roughness and stiffness of two polymer membranes on viability and mechanical properties of keratinocytes was studied. Terpolimer polyglicolide, polycaprolactone and polylactide, (PGA-PCL-PLA) and copolymer polycaprolactone, polyglicolide (PGA-PCL) substrates were used for membranes fabrication. Surface modification – the hydrolysis of the obtained membranes was carried out. The analysis of membranes’ surface properties revealed that RMS surface roughness and roughness factor of PGA-PCL-PLA membrane decreased after hydrolysis while its stiffness increased. In contrast, the PGA-PCL membrane stiffness was only slightly affected by NaOH treatment. Immortalized human keratinocytes (HaCaT) were grown under standard conditions on the surface of the studied membranes and characterized by means of atomic force microscopy and fluorescence microcopy. The results showed the substrate-dependent effect on cells’ properties.

Název v anglickém jazyce

The effect of polymeric membrane surface on HaCaT cell properties

Popis výsledku anglicky

The control of the surface properties is an important issue for applicability of polymer membranes interacting with cells. In this work, the influence of surface roughness and stiffness of two polymer membranes on viability and mechanical properties of keratinocytes was studied. Terpolimer polyglicolide, polycaprolactone and polylactide, (PGA-PCL-PLA) and copolymer polycaprolactone, polyglicolide (PGA-PCL) substrates were used for membranes fabrication. Surface modification – the hydrolysis of the obtained membranes was carried out. The analysis of membranes’ surface properties revealed that RMS surface roughness and roughness factor of PGA-PCL-PLA membrane decreased after hydrolysis while its stiffness increased. In contrast, the PGA-PCL membrane stiffness was only slightly affected by NaOH treatment. Immortalized human keratinocytes (HaCaT) were grown under standard conditions on the surface of the studied membranes and characterized by means of atomic force microscopy and fluorescence microcopy. The results showed the substrate-dependent effect on cells’ properties.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10610 - Biophysics

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Micron

ISSN

0968-4328

e-ISSN

1878-4291

Svazek periodika

101

Číslo periodika v rámci svazku

October

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

162-169

Kód UT WoS článku

000413283300022

EID výsledku v databázi Scopus

2-s2.0-85026432759