VISUALISATION OF BLOOD BRAIN BARRIER IMPAIRMENT AS EARLY MARKER OF POST STROKE EPILEPTOGENESIS

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21230%2F16%3A00306132" target="_blank" >RIV/68407700:21230/16:00306132 - isvavai.cz</a>

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1111/epi.13609/epdf" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/epi.13609/epdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/epi.13609" target="_blank" >10.1111/epi.13609</a>

Jazyk výsledku

angličtina

Název v původním jazyce

VISUALISATION OF BLOOD BRAIN BARRIER IMPAIRMENT AS EARLY MARKER OF POST STROKE EPILEPTOGENESIS

Popis výsledku v původním jazyce

Purpose: Emerging evidence suggest important role of the blood brain barrier (BBB) impairment in pathophysiology of post-stroke epilepsy. During period of BBB opening various blood derived compounds penetrate the brain tissue. They have been shown previously to have potential to induce acute symptomatic seizures, neuroinflammation and epileptogenesis. BBB assessment algorithms based on dynamic perfusion imaging are time and computationally demanding and inapplicable in preclinical CT imaging where dynamic scans are restricted by low speed acquisition. The aim of present study is to visualize regions of BBB impairment in both preclinical rodent models and human stroke patients using simple post-pre comparison. Method: To detect BBB impairment in patients suffering the stroke a T1 weighted MRI imaging was performed 7–10 days after the stroke without and with Gadolinium contrast. Visualization of BBB impairment in rat intraluminal MCAO model with reperfusion (90 min.) was performed using microCT scanner without and with application of nonionic iodine contrast 24 a 48 h after the reperfusion. Evans blue and tetrazolium staining was used to assess infarcted and albumin permeable area. CT and MRI datasets were processed using adapted post-pre comparison. Briefly, after the registration of images a statistically different pixels were found and the difference was weighted to signal levels of tissue with and without known blood tissue barrier (muscle and eye ball). Results: The post-pre comparison detected areas with significantly different signal which corresponded to those positive for Evans blue in rat MCAO. In majority of human MRI scans an area overlapping ischemic region and its surrounding was detected as region with enhanced Gd extravasation. Conclusion: The present study confirmed post-pre contrast comparison suitable for both clinical and preclinical visualization of BBB impairment after the stroke.

Název v anglickém jazyce

VISUALISATION OF BLOOD BRAIN BARRIER IMPAIRMENT AS EARLY MARKER OF POST STROKE EPILEPTOGENESIS

Popis výsledku anglicky

Purpose: Emerging evidence suggest important role of the blood brain barrier (BBB) impairment in pathophysiology of post-stroke epilepsy. During period of BBB opening various blood derived compounds penetrate the brain tissue. They have been shown previously to have potential to induce acute symptomatic seizures, neuroinflammation and epileptogenesis. BBB assessment algorithms based on dynamic perfusion imaging are time and computationally demanding and inapplicable in preclinical CT imaging where dynamic scans are restricted by low speed acquisition. The aim of present study is to visualize regions of BBB impairment in both preclinical rodent models and human stroke patients using simple post-pre comparison. Method: To detect BBB impairment in patients suffering the stroke a T1 weighted MRI imaging was performed 7–10 days after the stroke without and with Gadolinium contrast. Visualization of BBB impairment in rat intraluminal MCAO model with reperfusion (90 min.) was performed using microCT scanner without and with application of nonionic iodine contrast 24 a 48 h after the reperfusion. Evans blue and tetrazolium staining was used to assess infarcted and albumin permeable area. CT and MRI datasets were processed using adapted post-pre comparison. Briefly, after the registration of images a statistically different pixels were found and the difference was weighted to signal levels of tissue with and without known blood tissue barrier (muscle and eye ball). Results: The post-pre comparison detected areas with significantly different signal which corresponded to those positive for Evans blue in rat MCAO. In majority of human MRI scans an area overlapping ischemic region and its surrounding was detected as region with enhanced Gd extravasation. Conclusion: The present study confirmed post-pre contrast comparison suitable for both clinical and preclinical visualization of BBB impairment after the stroke.

Druh

O - Ostatní výsledky

CEP obor

JA - Elektronika a optoelektronika, elektrotechnika

OECD FORD obor

—

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů