TABLE-TOP WATER-WINDOW MICROSCOPE USING Z-PINCHING CAPILLARY DISCHARGE SOURCE

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21340%2F16%3A00301203" target="_blank" >RIV/68407700:21340/16:00301203 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68407700:21460/16:00301203 RIV/68407700:21340/19:00335870 RIV/68407700:21460/19:00335870

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

TABLE-TOP WATER-WINDOW MICROSCOPE USING Z-PINCHING CAPILLARY DISCHARGE SOURCE

Popis výsledku v původním jazyce

We present a design of a compact transmission water-window microscope based on nitrogen plasma induced by the Z-pinching capillary discharge. Nitrogen plasma is ideal for its quasi-monochromatic radiation with wavelength λ = 2.88 nm, which corresponds to the quantum transition 1s2-1s2p of helium-like nitrogen ion [1]. This wavelength falls within the so-called water-window wavelength region (λ = 2.3 – 4.4 nm) and thus provides the natural contrast between water and carbon-based substances, e.g. proteins. We present a characterization of the table-top Z-pinching capillary discharge source. The source was built in-house [2], plasma is generated by a current discharge through a 10 cm long, 3.2 mm inner diameter ceramic capillary filled with nitrogen gas. A ceramic capacitor bank with a maximum capacity of 21 nF is pulse charged by a Marx-Fitch generator up to 100 kV, and switched by self-breakdown spark-gap. The main current has a damped sinus shape, with half-period of 150 ns and maximum amplitude of 30 kA. Average photons flux is 5.5 x 1013 photons/(sr x line x pulse) and plasma spot has a circular shape with diameter 360 µm at full width half maximum. The spectrum of soft X-ray radiation produced by the source is filtered by titanium filter to achieve monochromatic radiation with wavelength λ = 2.88 nm. Filtered radiation is focused by an ellipsoidal nickel coated condenser mirror [3]. We used a Fresnel zone plate to create an image of a sample transmission on a SXR-sensitive CCD camera. To assess the resolution of the microscope, we imaged a standard sample – copper mesh - with magnification 300x and exposure time 1 min. The achieved spatial resolution is 110 nm at half-pitch. The images of Chrysodidymus cells are presented as well as the description of the sample preparation process.

Název v anglickém jazyce

TABLE-TOP WATER-WINDOW MICROSCOPE USING Z-PINCHING CAPILLARY DISCHARGE SOURCE

Popis výsledku anglicky

We present a design of a compact transmission water-window microscope based on nitrogen plasma induced by the Z-pinching capillary discharge. Nitrogen plasma is ideal for its quasi-monochromatic radiation with wavelength λ = 2.88 nm, which corresponds to the quantum transition 1s2-1s2p of helium-like nitrogen ion [1]. This wavelength falls within the so-called water-window wavelength region (λ = 2.3 – 4.4 nm) and thus provides the natural contrast between water and carbon-based substances, e.g. proteins. We present a characterization of the table-top Z-pinching capillary discharge source. The source was built in-house [2], plasma is generated by a current discharge through a 10 cm long, 3.2 mm inner diameter ceramic capillary filled with nitrogen gas. A ceramic capacitor bank with a maximum capacity of 21 nF is pulse charged by a Marx-Fitch generator up to 100 kV, and switched by self-breakdown spark-gap. The main current has a damped sinus shape, with half-period of 150 ns and maximum amplitude of 30 kA. Average photons flux is 5.5 x 1013 photons/(sr x line x pulse) and plasma spot has a circular shape with diameter 360 µm at full width half maximum. The spectrum of soft X-ray radiation produced by the source is filtered by titanium filter to achieve monochromatic radiation with wavelength λ = 2.88 nm. Filtered radiation is focused by an ellipsoidal nickel coated condenser mirror [3]. We used a Fresnel zone plate to create an image of a sample transmission on a SXR-sensitive CCD camera. To assess the resolution of the microscope, we imaged a standard sample – copper mesh - with magnification 300x and exposure time 1 min. The achieved spatial resolution is 110 nm at half-pitch. The images of Chrysodidymus cells are presented as well as the description of the sample preparation process.

Druh

O - Ostatní výsledky

CEP obor

BL - Fyzika plasmatu a výboje v plynech

OECD FORD obor

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Projekt

<a href="/cs/project/LTT17015" target="_blank" >LTT17015: Výzkum v rámci Mezinárodního centra hustého magnetizovaného plazmatu</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů