In-vitro study of therapeutic radionuclides’ impact on selected tissue and tumour cell lines
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21340%2F19%3A00334661" target="_blank" >RIV/68407700:21340/19:00334661 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
In-vitro study of therapeutic radionuclides’ impact on selected tissue and tumour cell lines
Popis výsledku v původním jazyce
Beta emitters are widely used in treatment of various oncological diseases for a long time. Alpha emitters belong to new and perspective candidates for therapeutic use and some of them have been already introduced into clinical practise. Targeted alpha-particle therapy (TAT) is a rapidly evolving field of cancer treatment. Nevertheless, there are some severe issues that prevent TAT from being a leading modality in radionuclide therapy. The nuclear recoil effect that causes the daughter nuclei release from the original radiopharmaceuticals is a critical problem for alpha emitters. Moreover, targeting and proper dosimetry is still an issue. Therefore, we focused on the dosimetry on cellular and subcellular level with an aim to quantitatively and qualitatively compare the effect of alpha and beta emitters on living cells. For our study we used Ra-223, Sm-153 and Re-186 as a model radionuclides. All radionuclides were used in the range 0-8 kBq/mL. Studied cell lines were V79 (Chinese hamster lung fibroblasts), DU145 (human adenocarcinoma cell line) and U87 (human primary glioblastoma cell line) obtained from American Type Culture Collection (ATCC). All cell lines have been cultivated in the presence of Ra-223, Sm-153 or Re-186 for 24 hours after the monolayer of the cells was created. After the cultivation with Ra-223, Sm-153 or Re-186, the clonogenic survival test was performed and survival curves for all cell lines were constructed. All obtained survival curves correspond to the linearly quadratic model. Sensitivity of both human carcinoma cell lines (adenocarcinoma and glioblastoma cell line) to treatment by all used radionuclides is higher than the sensitivity of the Chinese hamster pulmonary fibroblast cell line. Sensitivity of tested carcinoma or tissue cell lines is higher to alpha treatment than to beta treatment using the same applicated activities of alpha or beta emitters.
Název v anglickém jazyce
In-vitro study of therapeutic radionuclides’ impact on selected tissue and tumour cell lines
Popis výsledku anglicky
Beta emitters are widely used in treatment of various oncological diseases for a long time. Alpha emitters belong to new and perspective candidates for therapeutic use and some of them have been already introduced into clinical practise. Targeted alpha-particle therapy (TAT) is a rapidly evolving field of cancer treatment. Nevertheless, there are some severe issues that prevent TAT from being a leading modality in radionuclide therapy. The nuclear recoil effect that causes the daughter nuclei release from the original radiopharmaceuticals is a critical problem for alpha emitters. Moreover, targeting and proper dosimetry is still an issue. Therefore, we focused on the dosimetry on cellular and subcellular level with an aim to quantitatively and qualitatively compare the effect of alpha and beta emitters on living cells. For our study we used Ra-223, Sm-153 and Re-186 as a model radionuclides. All radionuclides were used in the range 0-8 kBq/mL. Studied cell lines were V79 (Chinese hamster lung fibroblasts), DU145 (human adenocarcinoma cell line) and U87 (human primary glioblastoma cell line) obtained from American Type Culture Collection (ATCC). All cell lines have been cultivated in the presence of Ra-223, Sm-153 or Re-186 for 24 hours after the monolayer of the cells was created. After the cultivation with Ra-223, Sm-153 or Re-186, the clonogenic survival test was performed and survival curves for all cell lines were constructed. All obtained survival curves correspond to the linearly quadratic model. Sensitivity of both human carcinoma cell lines (adenocarcinoma and glioblastoma cell line) to treatment by all used radionuclides is higher than the sensitivity of the Chinese hamster pulmonary fibroblast cell line. Sensitivity of tested carcinoma or tissue cell lines is higher to alpha treatment than to beta treatment using the same applicated activities of alpha or beta emitters.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
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OECD FORD obor
10402 - Inorganic and nuclear chemistry
Návaznosti výsledku
Projekt
<a href="/cs/project/NV16-30544A" target="_blank" >NV16-30544A: Nová vícefázová nanodiagnostika pro zobrazování nádorových onemocnění a predikci efektivity antiangiogenní terapie</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2019
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů