Interaction of BetaAmyloid with Membrane Investigated by Fluorescence Spectroscopy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F13%3A00213729" target="_blank" >RIV/68407700:21460/13:00213729 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Interaction of BetaAmyloid with Membrane Investigated by Fluorescence Spectroscopy

Popis výsledku v původním jazyce

Alzheimer?s disease is linked to the misfolding and aggregation of beta-amyloid (A?) peptides. The main mechanism of pathogenesis and the conditions which lead to uncontrolled self-assembly are unfortunately to this date not fully understood. Interactionof beta-amyloid with the neuronal membrane surface has been implicated to be a possible source of cytotoxicity. The objective of this thesis is the application of both time-resolved fluorescence spectroscopy and steady-state fluorescence spectroscopy tomonitor beta-amyloid aggregation and interactions with model biological membranes. A novel approach of monitoring beta-amyloid aggregation using the fluorescence lifetime of the dye Thioflavin T was employed. The experimental results bring new insightson fluorescence properties of Thioflavin T and on the utilisation of the dye for assessing the process of amyloid aggregation. The interaction of beta-amyloid with model membranes was studied via several spectroscopic methods using an env

Název v anglickém jazyce

Interaction of BetaAmyloid with Membrane Investigated by Fluorescence Spectroscopy

Popis výsledku anglicky

Alzheimer?s disease is linked to the misfolding and aggregation of beta-amyloid (A?) peptides. The main mechanism of pathogenesis and the conditions which lead to uncontrolled self-assembly are unfortunately to this date not fully understood. Interactionof beta-amyloid with the neuronal membrane surface has been implicated to be a possible source of cytotoxicity. The objective of this thesis is the application of both time-resolved fluorescence spectroscopy and steady-state fluorescence spectroscopy tomonitor beta-amyloid aggregation and interactions with model biological membranes. A novel approach of monitoring beta-amyloid aggregation using the fluorescence lifetime of the dye Thioflavin T was employed. The experimental results bring new insightson fluorescence properties of Thioflavin T and on the utilisation of the dye for assessing the process of amyloid aggregation. The interaction of beta-amyloid with model membranes was studied via several spectroscopic methods using an env

Druh

O - Ostatní výsledky

CEP obor

CF - Fyzikální chemie a teoretická chemie

OECD FORD obor

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Projekt

<a href="/cs/project/EE.2.3.20.0092" target="_blank" >EE.2.3.20.0092: Rozvoj výzkumného týmu BIO-OPT-XUV na FBMI ČVUT</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů