PVA Immunonanofibers with Controlled Decay

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F15%3A00240400" target="_blank" >RIV/68407700:21460/15:00240400 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68407700:21720/15:00240400 RIV/00216208:11130/15:10315697 RIV/61989592:15310/15:33156140 RIV/61989592:15110/15:33156140

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.polymer.2015.09.018" target="_blank" >http://dx.doi.org/10.1016/j.polymer.2015.09.018</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.polymer.2015.09.018" target="_blank" >10.1016/j.polymer.2015.09.018</a>

Jazyk výsledku

angličtina

Název v původním jazyce

PVA Immunonanofibers with Controlled Decay

Popis výsledku v původním jazyce

Nanofibers are utilized in crucial biomedical applications owing to their unique properties, including an enormous surface-to-volume ratio. Here, we report the biofunctionalization of polyvinyl alcohol (PVA) nanofibers by specific antibodies. This approach is built on regulated acylation of PVA nanofibers by polyethylene glycol with biotin (PEG-b) linker and sequence-specific binding of avidin–antibody conjugate. We have successfully demonstrated a chemically modified PVA-PEG-b, where the modification by the PEG-b linker resulted in the altered decay of nanofibers. Additionally, our studies on two different models indicate that the bound avidin–antibody conjugate was active. The first model was based on the binding of transferrin to immobilized anti-transferrin, and the second system was based on the recognition and binding of β1 integrin localized on mesenchymal stem cells resulting in their improved adhesion to PVA-PEG-b nanofibers. Our findings suggest that this is a versatile model for the preparation of PVA-based immuno-nanofibers whose decay can be controlled.

Název v anglickém jazyce

PVA Immunonanofibers with Controlled Decay

Popis výsledku anglicky

Nanofibers are utilized in crucial biomedical applications owing to their unique properties, including an enormous surface-to-volume ratio. Here, we report the biofunctionalization of polyvinyl alcohol (PVA) nanofibers by specific antibodies. This approach is built on regulated acylation of PVA nanofibers by polyethylene glycol with biotin (PEG-b) linker and sequence-specific binding of avidin–antibody conjugate. We have successfully demonstrated a chemically modified PVA-PEG-b, where the modification by the PEG-b linker resulted in the altered decay of nanofibers. Additionally, our studies on two different models indicate that the bound avidin–antibody conjugate was active. The first model was based on the binding of transferrin to immobilized anti-transferrin, and the second system was based on the recognition and binding of β1 integrin localized on mesenchymal stem cells resulting in their improved adhesion to PVA-PEG-b nanofibers. Our findings suggest that this is a versatile model for the preparation of PVA-based immuno-nanofibers whose decay can be controlled.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Polymer

ISSN

0032-3861

e-ISSN

—

Svazek periodika

77

Číslo periodika v rámci svazku

October

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

387-398

Kód UT WoS článku

000363484300045

EID výsledku v databázi Scopus

2-s2.0-84945485649