Comparison of three template preparation methods for routine detection of beak and feather disease virus and avian polyomavirus with single and nested polymerase chain reaction in clinical specimens.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70565813%3A_____%2F08%3A%230000117" target="_blank" >RIV/70565813:_____/08:#0000117 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of three template preparation methods for routine detection of beak and feather disease virus and avian polyomavirus with single and nested polymerase chain reaction in clinical specimens.

Popis výsledku v původním jazyce

Three DNA extraction methods were compared for routine polymerase chain reaction (PCR) detection of beak and feather disease virus (BFDV) in whole blood and feather samples and of avian polyomavirus (APV) in feather samples. Boiling in Chelex 100 Resin was found to be the most sensitive method for detection of BFDV or APV DNA in both feather and blood samples. The lower rate of BFDV DNA detection when using NucleoSpin kits was not a result of inhibition of PCR in most cases. Inhibition of DNA amplification was observed when using the DNAzol Direct kit. Therefore, of the methods evaluated here, Chelex 100 Resin treatment of samples was the best option for routine testing for BFDV and APV DNA in clinical samples.

Název v anglickém jazyce

Comparison of three template preparation methods for routine detection of beak and feather disease virus and avian polyomavirus with single and nested polymerase chain reaction in clinical specimens.

Popis výsledku anglicky

Three DNA extraction methods were compared for routine polymerase chain reaction (PCR) detection of beak and feather disease virus (BFDV) in whole blood and feather samples and of avian polyomavirus (APV) in feather samples. Boiling in Chelex 100 Resin was found to be the most sensitive method for detection of BFDV or APV DNA in both feather and blood samples. The lower rate of BFDV DNA detection when using NucleoSpin kits was not a result of inhibition of PCR in most cases. Inhibition of DNA amplification was observed when using the DNAzol Direct kit. Therefore, of the methods evaluated here, Chelex 100 Resin treatment of samples was the best option for routine testing for BFDV and APV DNA in clinical samples.

Druh

J_ost - Ostatní články v recenzovaných periodicích

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Avian pathology

ISSN

0307-9457

e-ISSN

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Svazek periodika

37

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

5

Strana od-do

145-149

Kód UT WoS článku

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EID výsledku v databázi Scopus

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