Using TaqMan Assay Followed By Melting Analysis Performed By New-Generation Intercalating Dyes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70565813%3A_____%2F13%3A%230000290" target="_blank" >RIV/70565813:_____/13:#0000290 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.researchgate.net/publication/249978429_qPCR__NGS_2013_Proceedings_6th_international_qPCR__NGS_Symposium_Industrial_Exhibition__Application_Workshops_Next_Generation_Thinking_in_Molecular_Diagnostics_Editor_Michael_W._Pfaffl_ISBN_9783000410246" target="_blank" >http://www.researchgate.net/publication/249978429_qPCR__NGS_2013_Proceedings_6th_international_qPCR__NGS_Symposium_Industrial_Exhibition__Application_Workshops_Next_Generation_Thinking_in_Molecular_Diagnostics_Editor_Michael_W._Pfaffl_ISBN_9783000410246</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Using TaqMan Assay Followed By Melting Analysis Performed By New-Generation Intercalating Dyes

Popis výsledku v původním jazyce

Hydrolysis probes, also known as TaqMan (Applied Biosystems), are routinely used in qPCR diagnostics. The disadvantage of these probes is that they have to be designed for each target sequence separately, which makes the analysis more expensive. In orderto reduce the costs, we can use the UPL probes (Universal ProbeLibrary; Roche), which contain locked nucleic acids and are of short length of just 8-9 nucleotides. The advantage of UPL probes is that they can bind to their targets with increased strength due to the incorporation of locked nucleic acids in their sequence. Their short length allows them to bind to several positions within the whole genome, which could be disadvantageous in some applications. Moreover, the point mutation can lead to the false negative or false positive results due to their short length. This problem can be overcome by using an intercalating dye which has a different fluorescent spectrum than UPL probe and binds unspecifically to any double stranded DNA Th

Název v anglickém jazyce

Using TaqMan Assay Followed By Melting Analysis Performed By New-Generation Intercalating Dyes

Popis výsledku anglicky

Hydrolysis probes, also known as TaqMan (Applied Biosystems), are routinely used in qPCR diagnostics. The disadvantage of these probes is that they have to be designed for each target sequence separately, which makes the analysis more expensive. In orderto reduce the costs, we can use the UPL probes (Universal ProbeLibrary; Roche), which contain locked nucleic acids and are of short length of just 8-9 nucleotides. The advantage of UPL probes is that they can bind to their targets with increased strength due to the incorporation of locked nucleic acids in their sequence. Their short length allows them to bind to several positions within the whole genome, which could be disadvantageous in some applications. Moreover, the point mutation can lead to the false negative or false positive results due to their short length. This problem can be overcome by using an intercalating dye which has a different fluorescent spectrum than UPL probe and binds unspecifically to any double stranded DNA Th

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/VF20112015013" target="_blank" >VF20112015013: Výzkum moderních metod detekce a identifikace nebezpečných CBRN látek a materiálů, metod snížení jejich nebezpečnosti a dekontaminace; výzkum moderních prostředků ochrany osob a prvků kritické infrastruktury</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

qPCR 2013 Symposium Proceedings

ISBN

978-3-00-041024-6

ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

52

Název nakladatele

TUM, Freising-Weihenstephan, Germany

Místo vydání

TUM, Freising-Weihenstephan, Germany

Místo konání akce

TUM, Weihenstephaner Berg 3, 85354 Freising

Datum konání akce

1. 1. 2013

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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