The effect of plasma pretreatment and cross-linking degree on antimicrobial activity of nisin coated film

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28110%2F19%3A63524304" target="_blank" >RIV/70883521:28110/19:63524304 - isvavai.cz</a>

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RIV/70883521:28610/19:63524304

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The effect of plasma pretreatment and cross-linking degree on antimicrobial activity of nisin coated film

Popis výsledku v původním jazyce

Stable antimicrobial nisin layers were prepared on the carrying medium - polyvinyl alcohol (PV A) films, crosslinked by glutaric acid. Surface plasma dielectric coplanar surface barrier discharge (DCSBD) modification of polyvinyl alcohol was used to improve the hydrophilic properties and to provide better adhesion of biologically active peptide - nisin to the polymer. The surface modification of films was studied in correlation to their cross-linking degree. Nisin was attached directly from the salt solution of commercial product. In order to achieve stable layer, the initial nisin concentration and following release was investigated by using chromatographic methods. Uniformity and stability of the layers was evaluated by means of zeta potential measurements and for surface changes of hydrophilic character the water contact angle measurements were provided. The nisin long term stability on the PV A films was confirmed by tricine polyacrylamide gel electrophoresis (SDS-PAGE) and by antimicrobial assay. It was proved that crosslinking degree of polyvinyl alcohol plays an important role mainly in the range of 10 - 20 %, which was evaluated as optimal for preparation of relatively water-stable peptide coatings that can sustain also the antibacterial properties with potential applicability in medical field. According to the zeta potential measurement, the adhesion proceeded at addic pH value and surface inhomogeneity was observed mainly in the case of 10% of crosslinking. This suggests that besides the bulk also the surface properties of the polymer materials must be carefully controlled to achieve acceptable results. Based on the results, DCSBD plasma treatment of PV A using 20% crosslinking with glutaric acid and nisin adhesion represents novel and promising method for design of biomaterials characterized by tunable stability and diffusion of bioactive compounds. It was found that PV A can serve as a suitable carrying medium for nisin with tunable properties by plasma treatment and crosslinking degree. Combination of coplanar Surface Barrier Discharge activation and cross-linking using environmentally friendly glutaric acid was applied to PV A to study its potential use as a carrier system for nisin adhesion. It was found that both mentioned factors have the crucial effect on the extent of nisin surface attachment and its release kinetics.

Název v anglickém jazyce

The effect of plasma pretreatment and cross-linking degree on antimicrobial activity of nisin coated film

Popis výsledku anglicky

Stable antimicrobial nisin layers were prepared on the carrying medium - polyvinyl alcohol (PV A) films, crosslinked by glutaric acid. Surface plasma dielectric coplanar surface barrier discharge (DCSBD) modification of polyvinyl alcohol was used to improve the hydrophilic properties and to provide better adhesion of biologically active peptide - nisin to the polymer. The surface modification of films was studied in correlation to their cross-linking degree. Nisin was attached directly from the salt solution of commercial product. In order to achieve stable layer, the initial nisin concentration and following release was investigated by using chromatographic methods. Uniformity and stability of the layers was evaluated by means of zeta potential measurements and for surface changes of hydrophilic character the water contact angle measurements were provided. The nisin long term stability on the PV A films was confirmed by tricine polyacrylamide gel electrophoresis (SDS-PAGE) and by antimicrobial assay. It was proved that crosslinking degree of polyvinyl alcohol plays an important role mainly in the range of 10 - 20 %, which was evaluated as optimal for preparation of relatively water-stable peptide coatings that can sustain also the antibacterial properties with potential applicability in medical field. According to the zeta potential measurement, the adhesion proceeded at addic pH value and surface inhomogeneity was observed mainly in the case of 10% of crosslinking. This suggests that besides the bulk also the surface properties of the polymer materials must be carefully controlled to achieve acceptable results. Based on the results, DCSBD plasma treatment of PV A using 20% crosslinking with glutaric acid and nisin adhesion represents novel and promising method for design of biomaterials characterized by tunable stability and diffusion of bioactive compounds. It was found that PV A can serve as a suitable carrying medium for nisin with tunable properties by plasma treatment and crosslinking degree. Combination of coplanar Surface Barrier Discharge activation and cross-linking using environmentally friendly glutaric acid was applied to PV A to study its potential use as a carrier system for nisin adhesion. It was found that both mentioned factors have the crucial effect on the extent of nisin surface attachment and its release kinetics.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

20506 - Coating and films

Projekt

<a href="/cs/project/GA17-10813S" target="_blank" >GA17-10813S: Nové plazmové polymery s laditelnou stabilitou a permeabilitou</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů