Morphological and physiological responses of in vitro-grown cucurbita sp. landraces seedlings under osmotic stress by mannitol and PEG

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28110%2F22%3A63555499" target="_blank" >RIV/70883521:28110/22:63555499 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.mdpi.com/2311-7524/8/12/1117" target="_blank" >https://www.mdpi.com/2311-7524/8/12/1117</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/horticulturae8121117" target="_blank" >10.3390/horticulturae8121117</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Morphological and physiological responses of in vitro-grown cucurbita sp. landraces seedlings under osmotic stress by mannitol and PEG

Popis výsledku v původním jazyce

Screening and identification of tolerant genotypes using osmotic materials under in vitro culture could be rapid, easy, and even accurate. In this research, three Iranian landraces of Cucurbita sp. included Tanbal Ajili (Cucurbita maxima Duch.), Ajili Razan (Cucurbita pepo L.), and Balghabakhi (Cucurbita moschata Duch.) seeds were cultured in 1/4 MS medium. After germination, plantlets were transferred to MS media containing mannitol and PEG 6000. Mannitol and PEG at three concentrations of 0.1, 0.2, and 0.4 M and 0.009, 0.012, and 0.015 M, respectively, were added into the MS medium, while the MS medium without any adding was used as control. Our findings revealed that osmotic treatments significantly increased shoot and root dry weight (DW), malondialdehyde (MDA), and proline content, but significantly reduced coleoptile length, shoot and root fresh weight, and photosynthesis pigments content. Protein content, phenols, and flavonoids content, enzymatic and non-enzymatic antioxidant including ascorbate peroxidase (APX), guaiacol peroxidase (GPX), superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR) activity, reduced ascorbate (AsA), reduced ascorbate/dehydroascorbic acid (AsA/DHA), reduced glutathione (GSH), dehydroascorbic acid (DHA) and oxidized glutathione (GSSG), and reduced glutathione/oxidized glutathione (GSH/GSSG) were significantly increased at moderate osmotic stress induced by mannitol and PEG. In contrast, the previous physiological parameters were significantly reduced at higher water deficit conditions. With respect to most attributes and concentrations, mannitol simulated osmotic stress better than PEG. Our results revealed that applying PEG and mannitol under in vitro conditions could be an efficient way to evaluate and screen cucurbit genotypes for future breeding programs.

Název v anglickém jazyce

Morphological and physiological responses of in vitro-grown cucurbita sp. landraces seedlings under osmotic stress by mannitol and PEG

Popis výsledku anglicky

Screening and identification of tolerant genotypes using osmotic materials under in vitro culture could be rapid, easy, and even accurate. In this research, three Iranian landraces of Cucurbita sp. included Tanbal Ajili (Cucurbita maxima Duch.), Ajili Razan (Cucurbita pepo L.), and Balghabakhi (Cucurbita moschata Duch.) seeds were cultured in 1/4 MS medium. After germination, plantlets were transferred to MS media containing mannitol and PEG 6000. Mannitol and PEG at three concentrations of 0.1, 0.2, and 0.4 M and 0.009, 0.012, and 0.015 M, respectively, were added into the MS medium, while the MS medium without any adding was used as control. Our findings revealed that osmotic treatments significantly increased shoot and root dry weight (DW), malondialdehyde (MDA), and proline content, but significantly reduced coleoptile length, shoot and root fresh weight, and photosynthesis pigments content. Protein content, phenols, and flavonoids content, enzymatic and non-enzymatic antioxidant including ascorbate peroxidase (APX), guaiacol peroxidase (GPX), superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR) activity, reduced ascorbate (AsA), reduced ascorbate/dehydroascorbic acid (AsA/DHA), reduced glutathione (GSH), dehydroascorbic acid (DHA) and oxidized glutathione (GSSG), and reduced glutathione/oxidized glutathione (GSH/GSSG) were significantly increased at moderate osmotic stress induced by mannitol and PEG. In contrast, the previous physiological parameters were significantly reduced at higher water deficit conditions. With respect to most attributes and concentrations, mannitol simulated osmotic stress better than PEG. Our results revealed that applying PEG and mannitol under in vitro conditions could be an efficient way to evaluate and screen cucurbit genotypes for future breeding programs.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Horticulturae

ISSN

2311-7524

e-ISSN

—

Svazek periodika

8

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

20

Strana od-do

nestrankovano

Kód UT WoS článku

000902721200001

EID výsledku v databázi Scopus

2-s2.0-85144882701