Polyethylene glycol and sorbitol-mediated in vitro screening for drought stress as an efficient and rapid tool to reach the tolerant cucumis melo l. genotypes
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28110%2F23%3A63566798" target="_blank" >RIV/70883521:28110/23:63566798 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/62156489:43210/23:43923236
Výsledek na webu
<a href="https://www.mdpi.com/2223-7747/12/4/870" target="_blank" >https://www.mdpi.com/2223-7747/12/4/870</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/plants12040870" target="_blank" >10.3390/plants12040870</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Polyethylene glycol and sorbitol-mediated in vitro screening for drought stress as an efficient and rapid tool to reach the tolerant cucumis melo l. genotypes
Popis výsledku v původním jazyce
An efficient method to instantly assess drought-tolerant plants after germination is using osmoregulation in tissue culture media. In this study, the responses of three Iranian melon genotypes to sorbitol (0.1, 0.2, and 0.4 M) or polyethylene glycol (PEG) (0.009, 0.012, and 0.015 M) were evaluated as drought stress simulators in MS medium. ‘Girke’ (GIR), ‘Ghobadloo’ (GHO), and ‘Toghermezi’ (TOG) were the genotypes. GIR is reputed as a drought-tolerant genotype in Iran. The PEG or sorbitol decreased the coleoptile length, fresh weight, and photosynthetic pigments content while enhancing proline and malondialdehyde (MDA) contents. Protein content and antioxidant enzyme activity were utterly dependent on genotype, osmotic regulators, and their concentration. Coleoptile length, root and shoot fresh weight, root dry weight, proline and MDA content, and guaiacol peroxidase (GPX) activity can be used as indicators for in vitro screening of Cucumis melo L. genotypes. The results showed that sorbitol mimics drought stress better than PEG. Overall, our findings suggest that in vitro screening could be an accurate, rapid, and reliable methodology for evaluating and identifying drought-tolerant genotypes.
Název v anglickém jazyce
Polyethylene glycol and sorbitol-mediated in vitro screening for drought stress as an efficient and rapid tool to reach the tolerant cucumis melo l. genotypes
Popis výsledku anglicky
An efficient method to instantly assess drought-tolerant plants after germination is using osmoregulation in tissue culture media. In this study, the responses of three Iranian melon genotypes to sorbitol (0.1, 0.2, and 0.4 M) or polyethylene glycol (PEG) (0.009, 0.012, and 0.015 M) were evaluated as drought stress simulators in MS medium. ‘Girke’ (GIR), ‘Ghobadloo’ (GHO), and ‘Toghermezi’ (TOG) were the genotypes. GIR is reputed as a drought-tolerant genotype in Iran. The PEG or sorbitol decreased the coleoptile length, fresh weight, and photosynthetic pigments content while enhancing proline and malondialdehyde (MDA) contents. Protein content and antioxidant enzyme activity were utterly dependent on genotype, osmotic regulators, and their concentration. Coleoptile length, root and shoot fresh weight, root dry weight, proline and MDA content, and guaiacol peroxidase (GPX) activity can be used as indicators for in vitro screening of Cucumis melo L. genotypes. The results showed that sorbitol mimics drought stress better than PEG. Overall, our findings suggest that in vitro screening could be an accurate, rapid, and reliable methodology for evaluating and identifying drought-tolerant genotypes.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
21101 - Food and beverages
Návaznosti výsledku
Projekt
—
Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2023
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Plants
ISSN
2223-7747
e-ISSN
—
Svazek periodika
12
Číslo periodika v rámci svazku
4
Stát vydavatele periodika
CH - Švýcarská konfederace
Počet stran výsledku
17
Strana od-do
—
Kód UT WoS článku
000941645900001
EID výsledku v databázi Scopus
2-s2.0-85149117396