Versatile Polysaccharide Hydrogels for Tissue Healing

Kód výsledku v IS VaVaI

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Nalezeny alternativní kódy

RIV/70883521:28610/25:63594503

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Versatile Polysaccharide Hydrogels for Tissue Healing

Popis výsledku v původním jazyce

The development of biomaterials capable of supporting tissue regeneration while minimizing inflammation is a major goal in regenerative medicine [1]. In this study, we present injectable, self-healing chitosan-based hydrogels crosslinked with dialdehyde cellulose (DAC) and incorporating polypyrrole (PPy) nanoparticles. These hydrogels are based on water-soluble, half-acetylated chitosan (SCN), prepared without synthetic modification, ensuring biocompatibility and solubility at physiological pH [2]. DAC enables both reversible Schiff base crosslinking and covalent anchoring of PPy colloids, resulting in a stable, shear-thinning hydrogel network [3].The materials were designed for application in wound healing across different tissue types, including bone and skin. Rheological analyses confirmed excellent injectability and rapid recovery after mechanical disruption, suitable for minimally invasive application. The hydrogels maintained cytocompatibility with fibroblasts and macrophages and exhibited consistent structural homogeneity.In the skin-related context, PPy-containing hydrogels promoted fibroblast migration in vitro and demonstrated strong anti-inflammatory properties by significantly reducing nitric oxide and interleukin-6 production in LPS-stimulated macrophages.For bone regeneration, BMP-2-loaded microspheres were incorporated into the hydrogel, enhancing osteogenic differentiation and repair in critical-size calvarial defects in vivo, while the presence of PPy reduced inflammation. In vivo implantation studies in mice demonstrated effective modulation of immune response and substantial bone regeneration, with the combination of PPy and BMP-2 yielding the highest bone repair percentages.These results demonstrate that the developed hydrogels represent a versatile platform for tissue healing, with potential applications in both orthopedic and dermal contexts. Their injectability, self-healing capability, anti-inflammatory action, and adaptability to tissue-specific modifications make them strong candidates for advanced wound healing therapies.

Název v anglickém jazyce

Versatile Polysaccharide Hydrogels for Tissue Healing

Popis výsledku anglicky

The development of biomaterials capable of supporting tissue regeneration while minimizing inflammation is a major goal in regenerative medicine [1]. In this study, we present injectable, self-healing chitosan-based hydrogels crosslinked with dialdehyde cellulose (DAC) and incorporating polypyrrole (PPy) nanoparticles. These hydrogels are based on water-soluble, half-acetylated chitosan (SCN), prepared without synthetic modification, ensuring biocompatibility and solubility at physiological pH [2]. DAC enables both reversible Schiff base crosslinking and covalent anchoring of PPy colloids, resulting in a stable, shear-thinning hydrogel network [3].The materials were designed for application in wound healing across different tissue types, including bone and skin. Rheological analyses confirmed excellent injectability and rapid recovery after mechanical disruption, suitable for minimally invasive application. The hydrogels maintained cytocompatibility with fibroblasts and macrophages and exhibited consistent structural homogeneity.In the skin-related context, PPy-containing hydrogels promoted fibroblast migration in vitro and demonstrated strong anti-inflammatory properties by significantly reducing nitric oxide and interleukin-6 production in LPS-stimulated macrophages.For bone regeneration, BMP-2-loaded microspheres were incorporated into the hydrogel, enhancing osteogenic differentiation and repair in critical-size calvarial defects in vivo, while the presence of PPy reduced inflammation. In vivo implantation studies in mice demonstrated effective modulation of immune response and substantial bone regeneration, with the combination of PPy and BMP-2 yielding the highest bone repair percentages.These results demonstrate that the developed hydrogels represent a versatile platform for tissue healing, with potential applications in both orthopedic and dermal contexts. Their injectability, self-healing capability, anti-inflammatory action, and adaptability to tissue-specific modifications make them strong candidates for advanced wound healing therapies.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů